神经性厌食症：L-精氨酸补充对心血管危险因素的作用？

Anorexia nervosa: a role for L-arginine supplementation in cardiovascular risk factors?

机构信息

Department of Biochemistry, Biology and Genetics, School of Medicine, Polytechnic University of Marche, Ancona, Italy.

出版信息

Int J Eat Disord. 2010 Jul;43(5):464-71. doi: 10.1002/eat.20709.

DOI:10.1002/eat.20709

PMID:19544555

Abstract

OBJECTIVE

The aim of the present study was to evaluate the effects of a short-term oral L-arginine treatment on platelet NO production, intracellular calcium concentration, iNOS and eNOS expression, in AN patients.

METHOD

Forty outpatients belonging to restricting subtype and 40 normal participants age and sex matched were enrolled in the study.

RESULTS

NO production was significantly elevated in the platelets from AN patients compared with controls while Ca(2+) was significantly decreased in patients with respect to controls. Western blot analysis demonstrated that iNOS isoform was more pronounced in the cell lysates from AN patients than controls. After supplementation with L-arginine, both NO production and Ca(2+) seem to return to control levels, suggesting a probable recovery of their metabolisms. The same was found after western blot analysis of NOS expression.

DISCUSSION

The results here proposed can be considered highly indicative of a positive effect of L-arginine supplementation on platelet NO production in AN patients.

摘要

目的

本研究旨在评估短期口服 L-精氨酸治疗对 AN 患者血小板一氧化氮（NO）生成、细胞内钙离子浓度、诱导型一氧化氮合酶（iNOS）和内皮型一氧化氮合酶（eNOS）表达的影响。

方法

将 40 名属于限制型亚型的门诊 AN 患者和 40 名年龄和性别匹配的正常参与者纳入研究。

结果

与对照组相比，AN 患者的血小板 NO 生成显著升高，而细胞内钙离子浓度（Ca(2+)）则显著降低。Western blot 分析表明，与对照组相比，AN 患者的 iNOS 同工型更为明显。补充 L-精氨酸后，NO 生成和Ca(2+)似乎恢复到对照水平，表明其代谢可能得到恢复。NOS 表达的 Western blot 分析也得到了同样的结果。