[中国四川地区人群、猪和鸡中戊型肝炎病毒的抗体检测及测序分析]
[Antibody detection and sequencing analysis of hepatitis E virus in human population, swine and chicken in Sichuan region in China].
作者信息
Jiang Yong-zhen, Lu Jian, Zhang Li-ping, Tian Rui-guang, Liu Qing-lian, Bi Sheng-li
机构信息
National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Dec;22(6):468-71.
OBJECTIVE
To investigate the seroprevalence of HEV infection and genotype.
METHODS
ELISA were used for detecting anti-HEV IgG of the serum samples, the nested reverse transcriptase PCR (RT-nPCR) was used for detecting HEV RNA in patient serum and swine bile samples. All samples were collected in 2005-2007 in some districts in Sichuan province. The primers used for genotyping were the ORF2 region of HEV genome.
RESULTS
The anti-HEV IgG was detected positive in childrens 6.10% (41/672), adults 42.26% (280/ 661), swines 88.89% (32/36), chickens negative (0/59). 1 case of 15 serum samples of anti-HEV IgM positive and 3 of 54 swine bile samples were positive for HEV RNA by RT-PCR.Sequence analysis of 4 isolates has 92.1% to 98.6% nucleotide sequence homology. These isolates from human and swine were identified closely related to Ch-T21 strain 90.1%-96.9% sequence homology, which belonged to HEV genotype 4B.
CONCLUSIONS
The swine were the risk factors in the spread of hepatitis E virus.
目的
调查戊型肝炎病毒（HEV）感染的血清流行率及基因型。
方法
采用酶联免疫吸附测定（ELISA）检测血清样本中的抗HEV IgG，采用巢式逆转录聚合酶链反应（RT - nPCR）检测患者血清和猪胆汁样本中的HEV RNA。所有样本于2005 - 2007年在四川省部分地区采集。用于基因分型的引物为HEV基因组的开放阅读框2（ORF2）区域。
结果
抗HEV IgG检测儿童阳性率为6.10%（41/672），成人阳性率为42.26%（280/661），猪阳性率为88.89%（32/36），鸡为阴性（0/59）。15份抗HEV IgM阳性血清样本中有1份、54份猪胆汁样本中有3份经RT - PCR检测HEV RNA呈阳性。4株分离株的序列分析显示核苷酸序列同源性为92.1%至98.6%。这些来自人和猪的分离株与Ch - T21株的序列同源性为90.1% - 96.9%，属于HEV基因型4B。
结论
猪是戊型肝炎病毒传播的危险因素。
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