Kongpol Ajiraporn, Pongtharangkul Thunyarat, Kato Junichi, Honda Kohsuke, Ohtake Hisao, Vangnai Alisa S
FEMS Microbiol Lett. 2009 Aug;297(2):225-33. doi: 10.1111/j.1574-6968.2009.01684.x. Epub 2009 Jun 6.
Brevibacillus agri strain 13 was isolated and characterized as a Gram-positive organic-solvent-tolerant bacterium able to grow at 45 degrees C. It can tolerate high concentrations (5% and 20%, v/v) of various organic solvents with a broad range of log P(ow) when the organic solvent was provided as a nonaqueous layer. Although it can tolerate a number of aromatic solvents, it cannot utilize them as a sole carbon source. The surface characteristics of cells exposed to organic solvent were investigated using the bacterial adhesion to hydrocarbon test, a contact angle measurement, zeta potential determination, and fluorescence microscopy analysis and compared with that of nonexposed cells. The results showed that although it has a hydrophilic cell surface, it has a unique indigenous cell surface characteristic in which the cells can stabilize solvent-in-water emulsion by adhering to the solvent-water interface of the solvent droplets. The tolerance and predilection of B. agri strain 13 toward organic solvents may suggest its potential application as a whole-cell biocatalyst for the biotransformation process of water-immiscible substrate(s).
从农业中分离出的短短芽孢杆菌菌株13被鉴定为革兰氏阳性耐有机溶剂细菌，能够在45摄氏度下生长。当有机溶剂以非水层形式提供时，它能耐受高浓度（5%和20%，v/v）的各种有机溶剂，且具有广泛的log P(ow)值。尽管它能耐受多种芳香族溶剂，但不能将它们作为唯一碳源利用。使用细菌对烃类的粘附试验、接触角测量、zeta电位测定和荧光显微镜分析研究了暴露于有机溶剂的细胞的表面特性，并与未暴露细胞的表面特性进行了比较。结果表明，尽管它具有亲水性细胞表面，但具有独特的固有细胞表面特性，即细胞可以通过粘附在溶剂液滴的溶剂 - 水界面上来稳定水包油乳液。短短芽孢杆菌菌株13对有机溶剂的耐受性和偏好可能表明其作为全细胞生物催化剂用于水不混溶底物生物转化过程的潜在应用。
