Segura A, Hurtado A, Rivera B, Lazaroaie M M
Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Granada, Spain.
J Appl Microbiol. 2008 May;104(5):1408-16. doi: 10.1111/j.1365-2672.2007.03666.x. Epub 2007 Dec 7.
To isolate and characterize new marine bacteria capable of tolerating high concentrations of organic solvents, and to understand the toxic effects of these chemicals on marine bacteria.
Five marine bacteria able to tolerate 0.1% (v/v) toluene were isolated and characterized on the basis of their growth and survival rates in the presence of different organic solvents. The toluene-tolerant marine bacteria identified in this study could not grow in the presence of 0.1% (v/v) of several organic solvents with a log P(ow) higher than that of the toluene (which in theory should be less toxic than toluene). The mechanisms underlying solvent tolerance were explored.
Isolates of four different genera were identified as toluene-tolerant. Toxicity of a second phase of an organic solvent toward these isolates could not be predicted on the basis of the solvents' log P(ow).
To improve the biodegradation rate of some water-insoluble compounds, double-phase bioreactors can be used. This type of bioreactor will require strains able to grow in a salt-containing environment and able to tolerate a second phase of an organic solvent.
分离并鉴定能够耐受高浓度有机溶剂的新型海洋细菌，并了解这些化学物质对海洋细菌的毒性作用。
分离出五种能够耐受0.1%（v/v）甲苯的海洋细菌，并根据它们在不同有机溶剂存在下的生长和存活率进行了鉴定。本研究中鉴定出的耐甲苯海洋细菌在存在0.1%（v/v）几种log P(ow)高于甲苯（理论上毒性应低于甲苯）的有机溶剂时无法生长。对溶剂耐受性的潜在机制进行了探索。
鉴定出四个不同属的菌株具有耐甲苯能力。不能根据有机溶剂的log P(ow)预测其对这些分离株的第二阶段毒性。
为提高某些水不溶性化合物的生物降解率，可使用双相生物反应器。这种类型的生物反应器将需要能够在含盐环境中生长并能耐受有机溶剂第二阶段的菌株。
