Ebert Susanne, Travis Kort, Lincoln Bryan, Guck Jochen
Opt Express. 2007 Nov 12;15(23):15493-9. doi: 10.1364/oe.15.015493.
The dual-beam laser trap is a versatile tool with many possible applications. In order to characterize its thermal properties in a microfluidic trap geometry we have developed a non-intrusive fluorescence ratio technique using the temperature sensitive dye Rhodamine B and the temperature independent reference dye Rhodamine 110. We measured temperature distribution profiles in the trap with submicron spatial resolution on a confocal laser-scanning microscope. The maximum heating in the center of the trap amounts to (13 +/- 2) degrees C/W for a wavelength of lambda = 1064 nm and scales linearly with the applied power. The measurements correspond well with simulated temperature distributions.
双光束激光阱是一种具有多种潜在应用的多功能工具。为了在微流体阱几何结构中表征其热特性，我们开发了一种非侵入式荧光比率技术，该技术使用对温度敏感的染料罗丹明B和与温度无关的参比染料罗丹明110。我们在共聚焦激光扫描显微镜上以亚微米空间分辨率测量了阱中的温度分布曲线。对于波长λ = 1064 nm，阱中心的最大加热量为(13±2)℃/W，并且与施加的功率呈线性比例关系。测量结果与模拟的温度分布非常吻合。
