Aswidinnoor H, Nelson R J, Dallas J F, McIntyre C L, Leung H, Gustafson J P
Division of Plant Pathology, International Rice Research Institute, Manila, Philippines.
Genome. 1991 Oct;34(5):790-8. doi: 10.1139/g91-123.
The value of genome-specific repetitive DNA sequences for use as molecular markers in studying genome differentiation was investigated. Five repetitive DNA sequences from wild species of rice were cloned. Four of the clones, pOm1, pOm4, pOmA536, and pOmPB10, were isolated from Oryza minuta accession 101141 (BBCC genomes), and one clone, pOa237, was isolated from Oryza australiensis accession 100882 (EE genome). Southern blot hybridization to different rice genomes showed strong hybridization of all five clones to O. minuta genomic DNA and no cross hybridization to genomic DNA from Oryza sativa (AA genome). The pOm1 and pOmA536 sequences showed cross hybridization only to all of the wild rice species containing the C genome. However, the pOm4, pOmPB10, and pOa237 sequences showed cross hybridization to O. australiensis genomic DNA in addition to showing hybridization to the O. minuta genomic DNA.
研究了基因组特异性重复DNA序列作为分子标记在研究基因组分化中的价值。从野生稻物种中克隆了五个重复DNA序列。其中四个克隆，即pOm1、pOm4、pOmA536和pOmPB10，是从小粒野生稻种质101141（BBCC基因组）中分离得到的，另一个克隆pOa237是从澳洲野生稻种质100882（EE基因组）中分离得到的。对不同水稻基因组进行Southern杂交分析表明，所有五个克隆均与小粒野生稻基因组DNA有强烈杂交信号，而与栽培稻（AA基因组）基因组DNA无交叉杂交信号。pOm1和pOmA536序列仅与所有含C基因组的野生稻物种有交叉杂交信号。然而，pOm4、pOmPB10和pOa237序列除了与小粒野生稻基因组DNA杂交外，还与澳洲野生稻基因组DNA有交叉杂交信号。
