Department of Agrobiology and Agrochemistry, University of Tuscia, I-01100, Viterbo, Italy.
Theor Appl Genet. 1995 Oct;91(5):738-43. doi: 10.1007/BF00220952.
The amount of alien chromatin introgressed in eight wheat/Ae. longissima Pm13 recombinant lines, involving breakpoints on the short arms of wheat chromosomes 3B and 3D, was evaluated by cytogenetic and molecular approaches. For each line the residual homologous synaptic ability of the recombinant chromosome in its proximal wheat and distal alien portion was estimated through meiotic analyses. Subsequently, telocentric and RFLP mapping were used to assess the genetic distance from the wheat centromere to the wheat/Ae. longissima breakpoints. One 3B recombinant line was distinguished from the other four by the chromosome pairing and telocentric mapping analyses. RFLP analysis succeeded in differentiating the remaining four lines into two groups. Chromosome pairing and telocentric mapping of the three 3D recombinant lines suggested that all had distinct breakpoints. However, the RFLP data could not discriminate between the two more proximal translocations. Physical locations for some RFLP loci were determined by a comparison of genotypes and C-banding karyotypes. This showed a considerable expansion of the genetic map compared to its physical length.
利用细胞遗传学和分子标记的方法评估了 8 个小麦/长穗偃麦草 Pm13 重组系中外源染色质的渗入量，这些重组系涉及到小麦染色体 3B 和 3D 短臂上的断点。通过减数分裂分析，估算了每个重组染色体近小麦和远外源部分的同源联会能力。随后，利用着丝粒作图和 RFLP 作图评估了从小麦着丝粒到小麦/长穗偃麦草断点的遗传距离。一个 3B 重组系与其他四个系的染色体配对和着丝粒作图分析结果不同。RFLP 分析成功地将其余四个系分为两组。三个 3D 重组系的染色体配对和着丝粒作图分析表明，它们都具有明显的断点。然而，RFLP 数据不能区分两个更近的易位。通过比较基因型和 C-带核型，确定了一些 RFLP 位点的物理位置。这表明与物理长度相比，遗传图谱有了相当大的扩展。
