单克隆抗体荧光偏振免疫分析法与¹²⁵I标记配体放射免疫分析法用于测定全血中环孢素浓度的比较

Monoclonal antibody fluorescent polarisation immunoassay versus 125Iode labelled ligand radioimmunoassay for the measurement of cyclosporine concentrations in whole blood.

作者信息

Montes C, Burtin P, Jacqz-Aigrain E

机构信息

Clinical Pharmacology Department, Hospital Robert-Debré, Paris, France.

出版信息

Fundam Clin Pharmacol. 1991;5(6):557-62. doi: 10.1111/j.1472-8206.1991.tb00742.x.

DOI:10.1111/j.1472-8206.1991.tb00742.x

PMID:1955199

Abstract

Monoclonal antibody fluorescent polarisation immunoassay for cyclosporine in whole blood was first evaluated. Inter- and intra-assay CVs were less than 7%. We also compared concentrations measured by 125I-RIA and FPIA in specimens obtained from kidney and bone marrow transplanted patients. FPIA correlated well with 125I-RIA (slope = 1.03, r = 0.989, n = 58) over a wide range of concentrations (44-984 ng/ml). However, an additive bias estimated by the mean difference in cyclosporine concentrations between the 2 readings (44.1 +/- 44.3 ng/ml), led to overestimation of cyclosporine concentrations measured by FPIA. The monoclonal FPIA kit is therefore a rapid and reproductive method to monitor cyclosporine concentrations in whole blood. However, FPIA and 125I-RIA are not interchangeable and the therapeutic range of cyclosporine measured by FPIA should be defined.

摘要

首次评估了全血中环孢素的单克隆抗体荧光偏振免疫测定法。批间和批内变异系数均小于7%。我们还比较了125I放射免疫分析法（RIA）和荧光偏振免疫分析法（FPIA）在肾移植和骨髓移植患者样本中测得的浓度。在很宽的浓度范围（44 - 984 ng/ml）内，FPIA与125I - RIA相关性良好（斜率 = 1.03，r = 0.989，n = 58）。然而，通过两次读数中环孢素浓度的平均差异估计的加性偏差（44.1 +/- 44.3 ng/ml）导致FPIA测得的环孢素浓度被高估。因此，单克隆FPIA试剂盒是监测全血中环孢素浓度的一种快速且重复性好的方法。然而，FPIA和125I - RIA不可互换，应由FPIA测定的环孢素治疗范围也应确定。