腹膜渗出巨噬细胞和肺泡巨噬细胞分泌弹性蛋白酶。
Elastase secretion by peritoneal exudative and alveolar macrophages.
作者信息
White R, Lin H S, Kuhn C
出版信息
J Exp Med. 1977 Sep 1;146(3):802-8. doi: 10.1084/jem.146.3.802.
Abstract
Mouse alveolar macrophages (AM) cultured in the absence of serum secrete an elastolytic enzyme. The elastase from AM resembles the previously described elastase from peritoneal macrophages (PM) in pH optimum and inhibition profile. The macrophage enzymes do not appear to be stored, and with periodic changes in the culture medium, accumulate extracellularly for up to 10 days. Resident PM produce barely detectable levels of extracellular elastase unless given a phagocytic load. Thioglycollate-stimulated peritoneal exudative macrophages (PEM), however, secret easily detectable levels of elastase, which can be further increased with a phagocytic load. Without any additional stimulation, AM secret an elastolytic activity comparable to that of the PEM receiving a phagocytic load, but unlike PM they do not increase elastase secretion after phagocytosis.
摘要
在无血清条件下培养的小鼠肺泡巨噬细胞（AM）分泌一种弹性蛋白酶。来自AM的弹性蛋白酶在最适pH值和抑制特性方面类似于先前描述的来自腹腔巨噬细胞（PM）的弹性蛋白酶。巨噬细胞酶似乎不是储存起来的，随着培养基的周期性变化，它们在细胞外积累长达10天。驻留的PM除非接受吞噬负荷，否则几乎检测不到细胞外弹性蛋白酶的水平。然而，经巯基乙酸盐刺激的腹腔渗出巨噬细胞（PEM）很容易分泌可检测水平的弹性蛋白酶，吞噬负荷可使其进一步增加。在没有任何额外刺激的情况下，AM分泌的弹性溶解活性与接受吞噬负荷的PEM相当，但与PM不同的是，它们在吞噬后不会增加弹性蛋白酶的分泌。
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