Hinman L M, Stevens C A, Matthay R A, Gee J B
Am Rev Respir Dis. 1980 Feb;121(2):263-71. doi: 10.1164/arrd.1980.121.2.263.
We compared the elastase and lysozyme activities of cells obtained by bronchoalveolar lavage from normal smokers and nonsmokers. After total and differential cell counts were obtained for the initial lavage cell population, we determined the enzyme activities of the total lavage cell population, the culture vessel's adherent alveolar macrophage cell fraction, and the cell culture supernatant medium. Our data indicated that macrophages, particularly from smokers, synthesized a calcium-dependent activity against a synthetic elastase substrate, succinyl-trialanine-p-nitroanilide. This activity was enhanced in smokers and was distinct from the polymorphonuclear leukocyte elastase as measured with this synthetic substrate. Measurements using insoluble elastin labeled with 3H demonstrated that smokers' macrophages also contained a serine-proteinase activity whose inhibitor profile resembled that of polymorphonuclear leukocyte elastase. Finally, macrophages from smokers secreted 5 times more lysozyme and contained more lactate dehydrogenase activity than did nonsmokers' macrophages. We suggest that pulmonary macrophages take up the polymorphonuclear leukocyte elastase and contain a synthetic substrate, "elastase". The biologic significance of this elastase activity is unclear. The enhanced lysozyme secretion by smokers' alveolar macrophages indicated increased biosynthetic activity by these cells.
我们比较了通过支气管肺泡灌洗从正常吸烟者和非吸烟者获得的细胞的弹性蛋白酶和溶菌酶活性。在对初始灌洗细胞群体进行总细胞计数和分类细胞计数后，我们测定了总灌洗细胞群体、培养容器中贴壁的肺泡巨噬细胞部分以及细胞培养上清培养基的酶活性。我们的数据表明，巨噬细胞，尤其是吸烟者的巨噬细胞，合成了一种针对合成弹性蛋白酶底物琥珀酰 - 丙氨酸 - 对硝基苯胺的钙依赖性活性。这种活性在吸烟者中增强，并且与用这种合成底物测量的多形核白细胞弹性蛋白酶不同。使用用³H标记的不溶性弹性蛋白进行的测量表明，吸烟者的巨噬细胞还含有一种丝氨酸蛋白酶活性，其抑制剂谱与多形核白细胞弹性蛋白酶相似。最后，吸烟者的巨噬细胞分泌的溶菌酶比非吸烟者的巨噬细胞多5倍，并且含有更多的乳酸脱氢酶活性。我们认为肺巨噬细胞摄取多形核白细胞弹性蛋白酶并含有一种合成底物“弹性蛋白酶”。这种弹性蛋白酶活性的生物学意义尚不清楚。吸烟者肺泡巨噬细胞溶菌酶分泌的增加表明这些细胞的生物合成活性增强。
