Kumar Puneet, Prasad Y, Patra A K, Ranjan R, Swarup D, Patra R C, Pal Satya
Aquatic Biotechnology and Fish Pathology Laboratory, Department of Animal Science, M.J.P. Rohilkhand University, Bareilly-243 006, India.
Sci Total Environ. 2009 Sep 1;407(18):5024-30. doi: 10.1016/j.scitotenv.2009.05.030. Epub 2009 Jun 23.
An experiment was conducted to investigate bioaccumulation potential of cadmium (Cd) and changes in oxidative stress indices in liver and kidney tissues from Cd-exposed catfish (Clarias batrachus) with or without simultaneous treatment of water with ascorbic acid, garlic extract or taurine. C. batrachus (n=324) with average length of 20+/-4 cm and weight of 86+/-5 g were used for the present investigation. Fishes were divided into nine groups (I to IX) each comprising 36 fishes. The fishes of groups II, III, IV and V were challenged with 5 ppm of cadmium chloride monohydrate (CdCl2.H2O), whereas groups VI, VII, VIII and IX were exposed to 10 ppm CdCl2.H2O solution for a period of 45 days. Group I was kept as negative control and the fishes of this group were maintained in water containing no added Cadmium. Group II and VI were maintained as Cd exposed non treated control to serve as positive controls. Fishes of III and VII, IV and VIII, V and IX received ascorbic acid (5 ppm), extract of dried garlic (5 ppm) or taurine (5 ppm), respectively during the entire experiment period. The concentrations of Cd in liver and kidney increased significantly following exposure to Cd and the level continued to rise with the increase in exposure duration. Treatment of tank water with ascorbic acid, garlic or taurine significantly reduced the Cd concentrations in tissues compared to the positive control group, but the level in Cd exposed groups was greater than the negative control group. Fishes exposed to Cd and treated with ascorbic acid, garlic or taurine had reduced oxidative stress as evidenced from lower concentration of lipid peroxides and higher activities of superoxide dismutase and catalase in liver, kidney and erythrocytes compared to fishes exposed to Cd. The reduction in Cd induced oxidative stress was highest in ascorbic acid treated group followed by garlic and taurine treatment. The results suggest that ascorbic acid, garlic and taurine have potential to reduce tissue accumulation of Cd and associated oxidative stress in freshwater catfish.
进行了一项实验，以研究镉（Cd）的生物累积潜力以及暴露于镉的鲶鱼（胡子鲶）肝脏和肾脏组织中氧化应激指标的变化，实验中鲶鱼在有或没有同时用抗坏血酸、大蒜提取物或牛磺酸处理水的情况下暴露于镉。本研究使用了平均体长为20±4厘米、体重为86±5克的胡子鲶（n = 324）。鱼被分为九组（I至IX），每组36条鱼。第二、三、四和五组的鱼用5 ppm的一水合氯化镉（CdCl₂·H₂O）进行挑战，而第六、七、八和九组的鱼暴露于10 ppm的CdCl₂·H₂O溶液中45天。第一组作为阴性对照，该组的鱼饲养在不添加镉的水中。第二组和第六组作为暴露于镉的未处理对照，作为阳性对照。第三组和第七组、第四组和第八组、第五组和第九组的鱼在整个实验期间分别接受抗坏血酸（5 ppm）、干大蒜提取物（5 ppm）或牛磺酸（5 ppm）。暴露于镉后，肝脏和肾脏中的镉浓度显著增加，并且随着暴露时间的增加水平持续上升。与阳性对照组相比，用抗坏血酸、大蒜或牛磺酸处理水箱水显著降低了组织中的镉浓度，但镉暴露组中的水平高于阴性对照组。与暴露于镉的鱼相比，暴露于镉并用抗坏血酸、大蒜或牛磺酸处理的鱼氧化应激降低，这从肝脏、肾脏和红细胞中较低的脂质过氧化物浓度以及较高的超氧化物歧化酶和过氧化氢酶活性得到证明。抗坏血酸处理组中镉诱导的氧化应激降低最高，其次是大蒜和牛磺酸处理。结果表明，抗坏血酸、大蒜和牛磺酸有潜力减少淡水鲶鱼组织中镉的积累以及相关的氧化应激。
