人巨细胞病毒毒株汤氏株和AD169株变体的高通量序列分析
High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169.
作者信息
Bradley Amanda J, Lurain Nell S, Ghazal Peter, Trivedi Urmi, Cunningham Charles, Baluchova Katarina, Gatherer Derek, Wilkinson Gavin W G, Dargan Derrick J, Davison Andrew J
机构信息
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Department of Immunology and Microbiology, Rush University Medical Center, 1653 West Congress Parkway, Chicago, IL 60612, USA.
出版信息
J Gen Virol. 2009 Oct;90(Pt 10):2375-2380. doi: 10.1099/vir.0.013250-0. Epub 2009 Jun 24.
The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.
人类巨细胞病毒（HCMV）毒株汤氏（Towne）和AD169常用变体的基因组各自都包含一个重大突变，即长独特区域右端的一个区域（U(L)/b'）被基因组左端一个区域的反向重复序列所取代。利用高通量技术，我们对HCMV毒株汤氏（ATCC VR - 977）进行了测序，并确认存在两种变体，一种在U(L)/b'区域出现了上述取代，另一种在该区域保持完整。两种变体在RL13、UL1、UL40、UL130、US1和US9基因中均发生了突变。我们还对一种新型AD169变体（varUC）进行了测序，该变体在U(L)/b'区域除了有一个影响UL144、UL142、UL141和UL140基因的小缺失外保持完整。与其他AD169变体一样，varUC在RL5A、RL13、UL36和UL131A基因中发生了突变。varUC的一个亚群包含一个额外的缺失，影响IRS1、US1和US2基因。
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