Sun Z-R, Ji Y-H, Ruan Q, He R, Ma Y-P, Qi Y, Mao Z-Q, Huang Y-J
Affiliated Shengjing Hospital, China Medical University, Shenyang, P. R. China.
Genet Mol Res. 2009 Sep 29;8(3):1191-201. doi: 10.4238/vol8-3gmr654.
Human cytomegalovirus (HCMV) genetic determinants of endothelial cell tropism, leukocytes and dendritic cells have been identified in the genes UL131A, UL130, and UL128. We examined the structure of these three genes in HCMV. Eighteen low-passage clinical isolates and five non-passage strains from congenitally HCMV-infected infants in China were used to assess the structures of the UL131A, UL130, and UL128 genes and to find possible relationships between sequence polymorphism and different signs of HCMV disease. Comparisons were made between the UL131A, UL130, and UL128 genes of clinical strains and published sequences of Towne and Merlin strains. The UL131A coding region in the clinical strains was similar to that of Towne and Merlin strains, while UL130, and UL128 coding regions in the clinical strains were parallel with those of Towne and Merlin, respectively. Sequence comparison indicated that the UL130, and UL128 genes encode chemokine-like proteins in the clinical strain; the transmembrane regions of UL131A, and UL130 were conserved in all clinical and reference strains. The three genes of clinical strains from infants with different signs of HCMV disease had similar structure characterization. We conclude that the UL131A, UL130, and UL128 genes are highly conserved in these clinical strains. No correlation was found between the structure of the three genes and variations in HCMV disease. The finding of chemokine-like domains in UL130, and UL128 putative proteins suggests that the predicted products play a role in HCMV infectivity.
人巨细胞病毒（HCMV）在内皮细胞嗜性、白细胞和树突状细胞方面的遗传决定因素已在UL131A、UL130和UL128基因中得到鉴定。我们研究了HCMV中这三个基因的结构。使用来自中国先天性HCMV感染婴儿的18株低代临床分离株和5株未传代菌株来评估UL131A、UL130和UL128基因的结构，并寻找序列多态性与HCMV疾病不同体征之间可能的关系。对临床菌株的UL131A、UL130和UL128基因与已发表的Towne和Merlin菌株序列进行了比较。临床菌株中的UL131A编码区与Towne和Merlin菌株相似，而临床菌株中的UL130和UL128编码区分别与Towne和Merlin菌株的编码区平行。序列比较表明，临床菌株中UL130和UL128基因编码趋化因子样蛋白；UL131A和UL130的跨膜区在所有临床和参考菌株中均保守。来自具有不同HCMV疾病体征婴儿的临床菌株的这三个基因具有相似的结构特征。我们得出结论，在这些临床菌株中，UL
