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MeningoFinder评估：一种用于同时检测六种引起中枢神经系统感染的病毒的新型多重连接依赖探针扩增检测方法。

Evaluation of MeningoFinder, a novel multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species causing central nervous system infections.

作者信息

Wolffs P F G, Vink C, Keijdener J, Habek B, Reijans M, Simons G, Bruggeman C A, van den Brule A J C

机构信息

Department of Medical Microbiology, Maastricht University Medical Center, The Netherlands.

出版信息

J Clin Microbiol. 2009 Aug;47(8):2620-2. doi: 10.1128/JCM.02436-08. Epub 2009 Jun 24.

DOI:10.1128/JCM.02436-08
PMID:19553572
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2725638/
Abstract

A multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species was developed and tested on clinical cerebrospinal fluid (CSF) samples. The assay, termed MeningoFinder, showed an accordance of 97%, concordance of 96%, interlaboratory sensitivity of 90%, and interlaboratory specificity of 94% compared to PCRs.

摘要

开发了一种用于同时检测六种病毒的多重连接依赖探针扩增检测方法，并在临床脑脊液（CSF）样本上进行了测试。该检测方法称为MeningoFinder，与聚合酶链反应（PCR）相比，一致性为97%，符合率为96%，实验室间灵敏度为90%，实验室间特异性为94%。

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本文引用的文献

1
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Neuroimaging Clin N Am. 2008 Feb;18(1):1-18; vii. doi: 10.1016/j.nic.2007.12.008.
2
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3
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J Clin Microbiol. 2007 Nov;45(11):3785-7. doi: 10.1128/JCM.01244-07. Epub 2007 Sep 19.
4
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5
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