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在外科心脏瓣膜标本中同时检测和区分五日热巴尔通体、亨氏巴尔通体和伯氏考克斯氏体的双重PCR检测法。

Duplex PCR assay simultaneously detecting and differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in surgical heart valve specimens.

作者信息

Tang Yi-Wei

机构信息

Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

出版信息

J Clin Microbiol. 2009 Aug;47(8):2647-50. doi: 10.1128/JCM.00721-09. Epub 2009 Jun 24.

DOI:10.1128/JCM.00721-09
PMID:19553582
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2725655/
Abstract

A duplex PCR (dPCR) assay was developed to simultaneously detect and differentiate Bartonella quintana, Bartonella henselae, and Coxiella burnetii from surgical heart valve tissue specimens with an analytic sensitivity of 10 copies/reaction. Among 17 specimens collected from patients with a clinical diagnosis of culture-negative endocarditis, 2, 4, and 2 were positive for B. quintana, B. henselae, and C. burnetii, respectively, by the dPCR assay, which matched the results obtained by universal bacterial 16S rRNA gene amplification and sequencing.

摘要

开发了一种双重PCR（dPCR）检测方法，用于从手术心脏瓣膜组织标本中同时检测和区分五日热巴尔通体、亨氏巴尔通体和伯氏考克斯氏体，分析灵敏度为10拷贝/反应。在从临床诊断为培养阴性心内膜炎的患者中收集的17份标本中，通过dPCR检测，分别有2份、4份和2份标本对五日热巴尔通体、亨氏巴尔通体和伯氏考克斯氏体呈阳性，这与通过通用细菌16S rRNA基因扩增和测序获得的结果相符。

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本文引用的文献

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