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使用一种基于多重PCR的新检测方法直接同时鉴定六种脑膜炎奈瑟菌血清群。

Use of a new single multiplex PCR-based assay for direct simultaneous characterization of six Neisseria meningitidis serogroups.

作者信息

Fraisier Christophe, Stor Richard, Tenebray Bernard, Sanson Yannick, Nicolas Pierre

机构信息

WHO Collaborating Centre, IRBA, Institut de Médecine Tropicale du Service de Santé des Armées, Marseille, France.

出版信息

J Clin Microbiol. 2009 Aug;47(8):2662-6. doi: 10.1128/JCM.02415-08. Epub 2009 Jun 24.

DOI:10.1128/JCM.02415-08
PMID:19553584
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2725692/
Abstract

We developed a new Neisseria meningitidis multiplex PCR to determine six serogroups, including X-specific primers, and to allow direct W135/Y discrimination. This assay offers a simple and low-cost method for serogrouping N. meningitidis from cerebrospinal fluid that could be useful in Africa.

摘要

我们开发了一种新的脑膜炎奈瑟菌多重PCR方法，用于确定包括X特异性引物在内的六个血清群，并实现W135/Y血清群的直接鉴别。该检测方法为从脑脊液中对脑膜炎奈瑟菌进行血清群分类提供了一种简单且低成本的方法，这在非洲可能会很有用。

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J Clin Microbiol. 2006 Mar;44(3):1127-31. doi: 10.1128/JCM.44.3.1127-1131.2006.
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PCR-based assay for detection of Neisseria meningitidis capsular serogroups 29E, X, and Z.基于聚合酶链反应的检测脑膜炎奈瑟菌29E、X和Z血清群的检测方法。
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Outbreaks of serogroup X meningococcal meningitis in Niger 1995-2000.1995 - 2000年尼日尔X群脑膜炎球菌性脑膜炎疫情
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