Eliott S, Vardy P H, Williams K L
School of Biological Sciences, Macquarie University, Sydney NSW, Australia.
J Cell Biol. 1991 Dec;115(5):1267-74. doi: 10.1083/jcb.115.5.1267.
While the role of myosin II in muscle contraction has been well characterized, less is known about the role of myosin II in non-muscle cells. Recent molecular genetic experiments on Dictyostelium discoideum show that myosin II is necessary for cytokinesis and multicellular development. Here we use immunofluorescence microscopy with monoclonal and polyclonal antimyosin antibodies to visualize myosin II in cells of the multicellular D. discoideum slug. A subpopulation of peripheral and anterior cells label brightly with antimyosin II antibodies, and many of these cells display a polarized intracellular distribution of myosin II. Other cells in the slug label less brightly and their cytoplasm displays a more homogeneous distribution of myosin II. These results provide insight into cell motility within a three-dimensional tissue and they are discussed in relation to the possible roles of myosin II in multicellular development.
虽然肌球蛋白II在肌肉收缩中的作用已得到充分表征，但人们对其在非肌肉细胞中的作用了解较少。最近对盘基网柄菌进行的分子遗传学实验表明，肌球蛋白II是胞质分裂和多细胞发育所必需的。在这里，我们使用单克隆和多克隆抗肌球蛋白抗体进行免疫荧光显微镜观察，以可视化多细胞盘基网柄菌蛞蝓细胞中的肌球蛋白II。外周和前部细胞的一个亚群用抗肌球蛋白II抗体标记明亮，并且这些细胞中的许多显示出肌球蛋白II的极化细胞内分布。蛞蝓中的其他细胞标记不太明亮，并且它们的细胞质显示出肌球蛋白II更均匀的分布。这些结果为三维组织内的细胞运动提供了见解，并结合肌球蛋白II在多细胞发育中的可能作用进行了讨论。
