• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过缺失阿霉素生物合成基因簇对产自变铅青链霉菌ATCC 27952的土臭素合酶进行探索。

Exploration of geosmin synthase from Streptomyces peucetius ATCC 27952 by deletion of doxorubicin biosynthetic gene cluster.

作者信息

Singh Bijay, Oh Tae-Jin, Sohng Jae Kyung

机构信息

Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University, # 100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.

出版信息

J Ind Microbiol Biotechnol. 2009 Oct;36(10):1257-65. doi: 10.1007/s10295-009-0605-0. Epub 2009 Jun 26.

DOI:10.1007/s10295-009-0605-0
PMID:19557446
Abstract

Thorough investigation of Streptomyces peucetius ATCC 27952 genome revealed a sesquiterpene synthase, named spterp13, which encodes a putative protein of 732 amino acids with significant similarity to S. avermitilis MA-4680 (SAV2163, GeoA) and S. coelicolor A3(2) (SCO6073). The proteins encoded by SAV2163 and SCO6073 produce geosmin in the respective strains. However, the spterp13 gene seemed to be silent in S. peucetius. Deletion of the doxorubicin gene cluster from S. peucetius resulted in increased cell growth rate along with detectable production of geosmin. When we over expressed the spterp13 gene in S. peucetius DM07 under the control of an ermE* promoter, 2.4 +/- 0.4-fold enhanced production of geosmin was observed.

摘要

对天蓝色链霉菌ATCC 27952基因组的深入研究发现了一种倍半萜合酶，命名为spterp13，它编码一种推定的由732个氨基酸组成的蛋白质，与阿维链霉菌MA-4680（SAV2163，GeoA）和天蓝色链霉菌A3(2)（SCO6073）具有显著相似性。SAV2163和SCO6073编码的蛋白质在各自的菌株中产生土腥味素。然而，spterp13基因在天蓝色链霉菌中似乎是沉默的。从天蓝色链霉菌中删除阿霉素基因簇导致细胞生长速率增加，同时可检测到土腥味素的产生。当我们在ermE*启动子的控制下在天蓝色链霉菌DM07中过表达spterp13基因时，观察到土腥味素的产量提高了2.4±0.4倍。

相似文献

1
Exploration of geosmin synthase from Streptomyces peucetius ATCC 27952 by deletion of doxorubicin biosynthetic gene cluster.通过缺失阿霉素生物合成基因簇对产自变铅青链霉菌ATCC 27952的土臭素合酶进行探索。
J Ind Microbiol Biotechnol. 2009 Oct;36(10):1257-65. doi: 10.1007/s10295-009-0605-0. Epub 2009 Jun 26.
2
Cloning and functional characterization of the germacradienol synthase (spterp13) from Streptomyces peucetius ATCC 27952.来自阿维链霉菌ATCC 27952的吉马烯醇合酶（spterp13）的克隆及功能表征
J Microbiol Biotechnol. 2008 Jul;18(7):1216-20.
3
Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase.阿维链霉菌中地霉烯素的生物合成。金合欢醇/地霉烯素合酶的分子克隆、表达及机制研究。
J Antibiot (Tokyo). 2006 Aug;59(8):471-9. doi: 10.1038/ja.2006.66.
4
Precursor for biosynthesis of sugar moiety of doxorubicin depends on rhamnose biosynthetic pathway in Streptomyces peucetius ATCC 27952.阿霉素糖部分生物合成的前体依赖于变铅青链霉菌 ATCC 27952 中的鼠李糖生物合成途径。
Appl Microbiol Biotechnol. 2010 Feb;85(5):1565-74. doi: 10.1007/s00253-009-2225-z. Epub 2009 Sep 24.
5
Doxorubicin overproduction in Streptomyces peucetius: cloning and characterization of the dnrU ketoreductase and dnrV genes and the doxA cytochrome P-450 hydroxylase gene.佩西链霉菌中阿霉素的过量生产：dnrU酮还原酶、dnrV基因和doxA细胞色素P-450羟化酶基因的克隆与特性分析
J Bacteriol. 1999 Jan;181(1):305-18. doi: 10.1128/JB.181.1.305-318.1999.
6
Isolation and genetic manipulation of the antibiotic down-regulatory gene, wblA ortholog for doxorubicin-producing Streptomyces strain improvement.阿霉素产生链霉菌的抗生素下调调控基因 wblA 同源物的分离和遗传操作及其用于菌株改良。
Appl Microbiol Biotechnol. 2010 Apr;86(4):1145-53. doi: 10.1007/s00253-009-2391-z. Epub 2009 Dec 18.
7
Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis.天蓝色链霉菌中与土臭素生物合成相关的牻牛儿烯醇合酶的表达及机制分析
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1547-51. doi: 10.1073/pnas.0337625100. Epub 2003 Jan 29.
8
Enhancement of doxorubicin production by expression of structural sugar biosynthesis and glycosyltransferase genes in Streptomyces peucetius.通过在变铅青链霉菌中表达结构糖生物合成和糖基转移酶基因提高阿霉素产量。
J Biosci Bioeng. 2009 Aug;108(2):92-8. doi: 10.1016/j.jbiosc.2009.03.002.
9
Identification and characterization of the afsR homologue regulatory gene from Streptomyces peucetius ATCC 27952.来自产紫链霉菌ATCC 27952的afsR同源调控基因的鉴定与表征
Res Microbiol. 2005 Jun-Jul;156(5-6):707-12. doi: 10.1016/j.resmic.2005.03.005. Epub 2005 Apr 21.
10
Genome analyses of Streptomyces peucetius ATCC 27952 for the identification and comparison of cytochrome P450 complement with other Streptomyces.对佩鲁茨链霉菌ATCC 27952进行基因组分析，以鉴定其细胞色素P450补体并与其他链霉菌进行比较。
Arch Biochem Biophys. 2004 May 15;425(2):233-41. doi: 10.1016/j.abb.2004.03.011.

引用本文的文献

1
A new peucemycin derivative and impacts of peuR and bldA on peucemycin biosynthesis in Streptomyces peucetius.一株新的佩枯菌素衍生物及其对佩枯菌素生物合成的影响。
Appl Microbiol Biotechnol. 2024 Dec;108(1):107. doi: 10.1007/s00253-023-12923-4. Epub 2024 Jan 12.
2
Actinomycetes as Producers of Biologically Active Terpenoids: Current Trends and Patents.作为生物活性萜类化合物生产者的放线菌：当前趋势与专利
Pharmaceuticals (Basel). 2023 Jun 12;16(6):872. doi: 10.3390/ph16060872.
3
Identification and enhancing production of a novel macrolide compound in engineered .

本文引用的文献

1
Genes and enzymes involved in bacterial isoprenoid biosynthesis.参与细菌类异戊二烯生物合成的基因和酶。
Curr Opin Chem Biol. 2009 Apr;13(2):180-8. doi: 10.1016/j.cbpa.2009.02.029. Epub 2009 Mar 21.
2
Cloning and functional characterization of the germacradienol synthase (spterp13) from Streptomyces peucetius ATCC 27952.来自阿维链霉菌ATCC 27952的吉马烯醇合酶（spterp13）的克隆及功能表征
J Microbiol Biotechnol. 2008 Jul;18(7):1216-20.
3
Identification and functional analysis of genes controlling biosynthesis of 2-methylisoborneol.
在工程菌中鉴定并提高一种新型大环内酯化合物的产量。
RSC Adv. 2021 Jan 14;11(5):3168-3173. doi: 10.1039/d0ra06099b. eCollection 2021 Jan 11.
4
Production of a Novel Tetrahydroxynaphthalene (THN) Derivative from sp. CS682 by Metabolic Engineering and Its Bioactivities.利用代谢工程从 sp. CS682 生产新型四羟基萘（THN）衍生物及其生物活性。
Molecules. 2019 Jan 10;24(2):244. doi: 10.3390/molecules24020244.
5
Metagenomic analysis of Streptomyces lividans reveals host-dependent functional expression.变形菌门 lividans 宏基因组分析揭示了宿主依赖性功能表达。
Appl Environ Microbiol. 2012 May;78(10):3622-9. doi: 10.1128/AEM.00044-12. Epub 2012 Mar 16.
6
Exploration and mining of the bacterial terpenome.细菌萜类化合物组的探索和挖掘。
Acc Chem Res. 2012 Mar 20;45(3):463-72. doi: 10.1021/ar200198d. Epub 2011 Oct 31.
控制2-甲基异冰片醇生物合成的基因的鉴定与功能分析。
Proc Natl Acad Sci U S A. 2008 May 27;105(21):7422-7. doi: 10.1073/pnas.0802312105. Epub 2008 May 20.
4
Geosmin biosynthesis. Mechanism of the fragmentation-rearrangement in the conversion of germacradienol to geosmin.土臭素的生物合成。金合欢烯醇转化为土臭素过程中的碎裂-重排机制。
J Am Chem Soc. 2008 Jan 16;130(2):428-9. doi: 10.1021/ja077792i. Epub 2007 Dec 21.
5
Studies on terpenoids produced by actinomycetes: oxaloterpins A, B, C, D, and E, diterpenes from Streptomyces sp. KO-3988.放线菌产生的萜类化合物研究：草酸萜类化合物A、B、C、D和E，链霉菌属KO - 3988产生的二萜类化合物。
J Nat Prod. 2007 Nov;70(11):1712-7. doi: 10.1021/np070326m. Epub 2007 Oct 31.
6
Analysis of intracellular short organic acid-coenzyme A esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry.利用液相色谱-电喷雾电离-质谱法分析放线菌中的细胞内短链有机酸辅酶A酯
J Mass Spectrom. 2007 Sep;42(9):1136-47. doi: 10.1002/jms.1240.
7
Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase.阿维链霉菌中地霉烯素的生物合成。金合欢醇/地霉烯素合酶的分子克隆、表达及机制研究。
J Antibiot (Tokyo). 2006 Aug;59(8):471-9. doi: 10.1038/ja.2006.66.
8
Engineering of primary carbohydrate metabolism for increased production of actinorhodin in Streptomyces coelicolor.通过改造初级碳水化合物代谢提高天蓝色链霉菌中放线紫红素的产量
Appl Environ Microbiol. 2006 Nov;72(11):7132-9. doi: 10.1128/AEM.01308-06. Epub 2006 Sep 1.
9
A gene cluster for biosynthesis of the sesquiterpenoid antibiotic pentalenolactone in Streptomyces avermitilis.阿维链霉菌中倍半萜类抗生素戊内酯生物合成的基因簇。
Biochemistry. 2006 May 16;45(19):6179-86. doi: 10.1021/bi060419n.
10
Biosynthesis of the terpene phenalinolactone in Streptomyces sp. Tü6071: analysis of the gene cluster and generation of derivatives.链霉菌属菌株Tü6071中萜类苯并呋喃内酯的生物合成：基因簇分析及衍生物的生成
Chem Biol. 2006 Apr;13(4):365-77. doi: 10.1016/j.chembiol.2006.01.011.