Dippold W, Meyer zum Büschenfelde K H
Department of Internal Medicine I, Johannes-Gutenberg-Universität, Mainz, FRG.
Immunol Res. 1991;10(3-4):258-60. doi: 10.1007/BF02919703.
Hematopoietic growth factors have been well characterized by cDNA cloning in recent years. In order to determine the influence of rhGM-CSF and rhIL-3 on epithelial cells of the gastrointestinal tract, their influence on in vitro cultured gastric and pancreas cancer cells was determined. A more than two-fold enhancement of proliferation was observed by IL-3 and GM-CSF in Mz-Sto-1 gastric and 818-4 pancreas carcinoma cells, applying a sensitive microculture system which allows precise quantification. The highest growth rates were obtained adding 1-10 ng/ml of the growth factors, but even picogram amounts were effective. Expression of mRNA for GM-CSF and IL-3 remained undetectable in the cell lines, but mRNA for the EGF receptor was demonstrated. However, IL-3 and GM-CSF did not up-regulate mRNA for the EGF receptor. Therefore, the hematopoietic growth factors IL-3 and GM-CSF stimulate proliferation of epithelial cells derived from gastric and pancreas carcinomas by a paracrine mechanism. This effect did not involve an up-regulation of EGF receptor mRNA.
近年来，造血生长因子已通过cDNA克隆得到了充分表征。为了确定重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）和重组人白细胞介素-3（rhIL-3）对胃肠道上皮细胞的影响，研究了它们对体外培养的胃癌和胰腺癌细胞的影响。采用一种灵敏的微量培养系统（可进行精确量化），观察到IL-3和GM-CSF可使Mz-Sto-1胃癌细胞和818-4胰腺癌细胞的增殖增强两倍以上。添加1-10 ng/ml的生长因子可获得最高生长速率，但即使是皮克量也有效。在这些细胞系中未检测到GM-CSF和IL-3的mRNA表达，但检测到了表皮生长因子（EGF）受体的mRNA。然而，IL-3和GM-CSF并未上调EGF受体的mRNA。因此，造血生长因子IL-3和GM-CSF通过旁分泌机制刺激源自胃癌和胰腺癌的上皮细胞增殖。这种作用不涉及EGF受体mRNA的上调。
