Dippold W G, Kron G, Boosfeld E, Dienes H P, Klingel R, Knuth A, Wagner R, Meyer Zum Büschenfelde K H
Department of Internal Medicine, Johannes Gutenberg Universität, Mainz, W. Germany.
Eur J Cancer Clin Oncol. 1987 Jun;23(6):697-706. doi: 10.1016/0277-5379(87)90265-3.
A human gastric signet ring cancer cell line (Mz-Sto-1) was established in tissue culture from the ascites fluid of a 54-year-old patient. The tumor cells growing in tissue culture exhibit the morphological characteristics of signet ring cells in phase contrast and transmission electron microscopy. Mz-Sto-1 cells grow as monolayer with a population doubling time of 28-36 hr during exponential growth phase and show a chromosome number between 72 and 74. In the cellular DNA of Mz-Sto-1 cells no amplification of 19 oncogenes studied is observed, c-myc included. Mz-Sto-1 cells secrete 150-250 ng CEA per 10(7) cell in 3 days, but no AFP. In addition Mz-Sto-1 cells and 2 already established gastric cancer cell lines MKN-28 and MKN-45 express HLA- and blood group related antigens (A, Lewis). HLA-DR antigens, which are regularly detected on normal stomach epithelium, are not found on any of the 3 cultured gastric cell lines. Mz-Sto-1 cells represent the first human gastric cancer cell characterized ultrastructurally as signet ring cells. This line will be a valuable tool to study the biology and genetics of gastric carcinoma, to test cytostatic drugs and to define new antigenic markers for stomach cancer.
从一名54岁患者的腹水中通过组织培养建立了人胃印戒癌细胞系（Mz-Sto-1）。在组织培养中生长的肿瘤细胞在相差显微镜和透射电子显微镜下呈现印戒细胞的形态特征。Mz-Sto-1细胞呈单层生长，在指数生长期的群体倍增时间为28 - 36小时，染色体数在72至74之间。在Mz-Sto-1细胞的细胞DNA中，未观察到所研究的19种癌基因（包括c-myc）的扩增。Mz-Sto-1细胞每10⁷个细胞在3天内分泌150 - 250 ng癌胚抗原（CEA），但不分泌甲胎蛋白（AFP）。此外，Mz-Sto-1细胞以及另外两种已建立的胃癌细胞系MKN-28和MKN-45表达与人类白细胞抗原（HLA）和血型相关的抗原（A、Lewis）。在正常胃上皮细胞上经常检测到的HLA-DR抗原，在这三种培养的胃细胞系中均未发现。Mz-Sto-1细胞是首个在超微结构上被鉴定为印戒细胞的人胃癌细胞系。该细胞系将成为研究胃癌生物学和遗传学、测试细胞抑制药物以及确定胃癌新抗原标志物的宝贵工具。
