Lindahl R
Biochem J. 1977 Apr 15;164(1):119-23. doi: 10.1042/bj1640119.
The pre- and post-natal ontogeny of Sprague-Dawley rat liver aldehyde dehydrogenase [aldehyde-NAD(P)(+) oxidoreductase, EC 1.2.1.5] is described. At no time in its ontogenetic development does normal liver aldehyde dehydrogenase exhibit any of the characteristics of a series of unique aldehyde dehydrogenases that can be isolated from 2-acetamidofluorene-induced rat hepatomas. Enzyme activity is first detectable in 15-day foetal liver and gradually increases throughout pre- and post-natal development until adult activities are attained by day 49 after birth. Electrophoretically, normal aldehyde dehydrogenase, throughout its ontogeny, exists as the same single isoenzyme found in normal adult liver. Isoelectric points for two normal liver isoenzymes demonstrable by isoelectric focusing are pH5.9 and 6.0. The immunochemical properties of aldehyde dehydrogenase during its ontogeny are identical with those of normal adult liver aldehyde dehydrogenase when tested against anti-(hepatoma aldehyde dehydrogenase) serum in Ouchterlony double-diffusion tests. The results indicate that the hepatoma-specific aldehyde dehydrogenases are not the result of the de-repression of genes normally repressed in adult rat liver or in some other adult tissue.
本文描述了Sprague-Dawley大鼠肝脏醛脱氢酶[醛-NAD(P)(+)氧化还原酶，EC 1.2.1.5]在产前和产后的个体发育过程。在其个体发育过程中的任何时候，正常肝脏醛脱氢酶都不表现出一系列可从2-乙酰氨基芴诱导的大鼠肝癌中分离出的独特醛脱氢酶的任何特征。酶活性最早在15日龄胎儿肝脏中可检测到，并在产前和产后发育过程中逐渐增加，直至出生后第49天达到成年活性。在整个个体发育过程中，正常醛脱氢酶通过电泳表现为与正常成年肝脏中发现的相同单一同工酶。通过等电聚焦可证明的两种正常肝脏同工酶的等电点分别为pH5.9和6.0。在Ouchterlony双向扩散试验中，用抗（肝癌醛脱氢酶）血清检测时，醛脱氢酶在其个体发育过程中的免疫化学性质与正常成年肝脏醛脱氢酶相同。结果表明，肝癌特异性醛脱氢酶不是成年大鼠肝脏或其他成年组织中正常被抑制基因去抑制的结果。
