编码内鞭毛亚基蛋白的问号钩端螺旋体基因的分子分析。
Molecular analysis of a Leptospira borgpetersenii gene encoding an endoflagellar subunit protein.
作者信息
Mitchison M, Rood J I, Faine S, Adler B
机构信息
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
出版信息
J Gen Microbiol. 1991 Jul;137(7):1529-36. doi: 10.1099/00221287-137-7-1529.
A flagellin gene, flaB, from Leptospira borgpetersenii (formerly L. interrogans) serovar hardjo was cloned and expressed in Escherichia coli. Expression of the 32 kDa FlaB protein was dependent upon the lacZ promoter from pUC18. Nucleotide sequence data showed an open reading frame encoding 283 amino acid residues, corresponding to a protein of molecular mass 31.3 kDa. The G + C content of the flaB gene was 54.7 mol%. Comparison of the deduced FlaB amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Treponema pallidum FlaB proteins.
来自博氏疏螺旋体（以前称为问号钩端螺旋体）血清型哈德乔的鞭毛蛋白基因flaB在大肠杆菌中被克隆并表达。32 kDa FlaB蛋白的表达依赖于pUC18的lacZ启动子。核苷酸序列数据显示一个开放阅读框，编码283个氨基酸残基，对应于分子量为31.3 kDa的蛋白质。flaB基因的G + C含量为54.7 mol%。将推导的FlaB氨基酸序列与其他细菌的鞭毛蛋白进行比较，发现与梅毒螺旋体的FlaB蛋白具有高度同源性。
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