美沙拉嗪或大肠杆菌 Nissle 1917 对溃疡性结肠炎中微卫星不稳定性的体内作用。
In vivo effects of mesalazine or E. coli Nissle 1917 on microsatellite instability in ulcerative colitis.
机构信息
Division of Gastroenterology, Department of Internal Medicine and Charles A Sammons Cancer Center, Baylor University Medical Center, Dallas, TX 75246, USA.
出版信息
Aliment Pharmacol Ther. 2009 Sep 15;30(6):634-42. doi: 10.1111/j.1365-2036.2009.04076.x. Epub 2009 Jun 25.
BACKGROUND
Microsatellite instability (MSI) occurs in chronically inflamed colorectal tissue and may evolve to colitis-associated cancer. In vitro data suggest that mesalazine (5-ASA) improves MSI.
AIM
To analyse the changes in MSI in 156 distal colonic biopsies of 39 patients with ulcerative colitis that had been treated within a randomized, double-blind trial comparing 5-ASA with E. coli Nissle (EcN).
METHODS
Two biopsies had been collected before and after 1 year of treatment. MSI testing was performed using a panel of eight markers, including 3 dinucleotide and 5 mononucleotide repeats.
RESULTS
No MSI was observed with any of the mono-repeats, and among dinucleotide repeats, only D5S346 (maps to APC) and D17S250 (p53) were consistently informative. Overall, 31/156 (20%) biopsies displayed MSI. After 1 year, 3/11 patients displayed MSI improvement [change to microsatellite stability (MSS); 1 on 5-ASA, 2 on EcN] at D5S346 and 4/11 showed MSI worsening (change from MSS to MSI; all 5-ASA). For D17S250, the corresponding data were for 3/9 patients (2 on 5-ASA, 1 on EcN) and 2/9 (both on 5-ASA), respectively.
CONCLUSIONS
In the set of biopsies taken from patients treated with 1.5 g 5-ASA for 1 year, there was no improvement in the prevalence of MSI in the distal colon.
背景
微卫星不稳定性 (MSI) 发生在慢性炎症性结直肠组织中，并可能发展为结肠炎相关癌症。体外数据表明美沙拉嗪（5-ASA）可改善 MSI。
目的
分析在溃疡性结肠炎患者的 156 例远端结肠活检中 MSI 的变化，这些患者曾参与一项随机、双盲试验，比较 5-ASA 与大肠杆菌 Nissle（EcN）的疗效。
方法
在治疗 1 年后收集了 2 次活检。使用包含 3 个二核苷酸和 5 个单核苷酸重复的 8 个标记物的面板进行 MSI 检测。
结果
任何单核苷酸重复均未观察到 MSI，在二核苷酸重复中，只有 D5S346（映射至 APC）和 D17S250（p53）始终具有信息性。总体而言，156 个活检中有 31 个显示 MSI。治疗 1 年后，3/11 名患者的 D5S346 处 MSI 改善（从微卫星不稳定（MSS）转为 MSS；5-ASA 治疗组 1 例，EcN 治疗组 2 例），4/11 例显示 MSI 恶化（从 MSS 转为 MSI；均为 5-ASA 治疗组）。对于 D17S250，相应的数据为 3/9 名患者（5-ASA 治疗组 2 例，EcN 治疗组 1 例）和 2/9 名患者（均为 5-ASA 治疗组）。
结论
在接受 1.5 g 5-ASA 治疗 1 年的患者中，远端结肠 MSI 的发生率没有改善。
Zotero 插件