School of Life Sciences, Kingston University, Kingston upon Thames, Surrey, UK.
FEMS Microbiol Lett. 2009 Aug;297(1):10-6. doi: 10.1111/j.1574-6968.2009.01654.x. Epub 2009 May 16.
Eight novel chromogenic substrates were evaluated for their efficacy in detecting lipase activity in clinical isolates of Staphylococcus aureus from the United Kingdom and Malta. All isolates metabolized the chromogenic lipase substrates 5-(4-hydroxy-3,5-dimethoxyphenylmethylene)-2-thioxothia-zolidin-4-one-3-ethanoic acid (SRA)-propionate, SRA-butyrate, SRA-octanoate and 2-[2-(4-hydroxy-3,5-dimethoxyphenyl)-vinyl]-3-methy-benzothiazolium salt (SB(Z)TM)-acetate. Over 90% of the isolates metabolized the lipase substrates SRA-decanoate and SRA-laurate. However, only 0.6% of UK isolates and 2% of Maltese isolates metabolized the lipase substrate SRA-myristate; none of the isolates tested metabolized SB(Z)TM-butyrate. Traditional Tween 80 assays showed that over 73% of the UK methicillin-resistant Staphylococcus aureus (MRSA) isolates and 83% of the UK methicillin-sensitive Staphylococcus aureus (MSSA) isolates demonstrated lipolytic activity. In contrast, Maltese isolates showed lipase activity in 94% and 88% of the MRSA and MSSA strains, respectively. Lipases in MRSA and MSSA demonstrated substrate specificity whose activity appeared dependent upon hydrocarbon chain length of the chromogen. These novel chromogens can be used for lipase enzyme detection and have application for full characterization of numerous S. aureus lipases.
八种新型显色底物用于检测来自英国和马耳他的金黄色葡萄球菌临床分离株的脂肪酶活性。所有分离株都代谢了显色脂肪酶底物 5-(4-羟基-3,5-二甲氧基苯基亚甲基)-2-硫代噻唑啉-4-酮-3-乙醇酸（SRA）-丙酸酯、SRA-丁酸酯、SRA-辛酸酯和 2-[2-(4-羟基-3,5-二甲氧基苯基）-乙烯基]-3-甲基苯并噻唑鎓盐（SB（Z）TM）-乙酸盐。超过 90%的分离株代谢脂肪酶底物 SRA-癸酸酯和 SRA-月桂酸酯。然而，只有 0.6%的英国分离株和 2%的马耳他分离株代谢脂肪酶底物 SRA-肉豆蔻酸酯；没有分离株测试代谢 SB（Z）TM-丁酸酯。传统的吐温 80 测定表明，超过 73%的英国耐甲氧西林金黄色葡萄球菌（MRSA）分离株和 83%的英国甲氧西林敏感金黄色葡萄球菌（MSSA）分离株表现出脂肪酶活性。相比之下，马耳他分离株的 MRSA 和 MSSA 菌株分别有 94%和 88%表现出脂肪酶活性。MRSA 和 MSSA 中的脂肪酶表现出底物特异性，其活性似乎取决于发色团的烃链长度。这些新型显色剂可用于脂肪酶酶检测，并可用于对大量金黄色葡萄球菌脂肪酶进行全面表征。
