紫癜性肾炎患儿血管内皮生长因子的基因多态性

Gene polymorphism of vascular endothelial growth factor in children with Henoch-Schonlein purpura nephritis.

作者信息

Zeng Hua-Song, Xiong Xiao-Yan, Chen Yao-Yong, Luo Xiao-Ping

机构信息

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2009 Jun;11(6):417-21.

PMID:19558800

Abstract

OBJECTIVE

To study the relationship of -634G/C gene polymorphism of vascular endothelial growth factor (VEGF) with Henoch-Schonlein purpura nephritis (HSPN) in children.

METHODS

One hundred ethnic Han children with HSP, including 50 children with concurrent nephritis (HSPN group) and 50 children without nephritis (HSP without nephritis group), were enrolled. Fifty age-, sex-and ethnics-matched healthy children were used as the control group. VEGF-634G/C genotypes were determined by PCR-RFLP. Plasma VEGF levels were measured using ELISA.

RESULTS

CC genotype distribution (32%) and C allele frequency (56%) in the HSPN group were significantly higher than those in the control group (10% and 35% respectively) and the HSP without nephritis group (10% and 33% respectively) (P<0.01). The incidence of nephritis in HSP patients with CC genotype increased significantly when compared with those with GG genotype (76% vs 31%; P<0.01). Plasma VEGF levels in patients with CC genotype (180.5+/- 40.7 pg/mL) were significantly higher than those in patients with CG (145.2+/- 48.3 pg/mL) and GG (101.5+/- 26.5 pg/mL) genotypes (P<0.05).

CONCLUSIONS

VEGF-634G/C gene polymorphism may be associated with the development of HSPN. C allele may a susceptible gene of HSPN.

摘要

目的

研究血管内皮生长因子（VEGF）-634G/C基因多态性与儿童过敏性紫癜性肾炎（HSPN）的关系。

方法

纳入100例汉族过敏性紫癜患儿，其中并发肾炎的患儿50例（HSPN组），未并发肾炎的患儿50例（非肾炎性过敏性紫癜组）。选取50例年龄、性别和种族相匹配的健康儿童作为对照组。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测VEGF-634G/C基因分型。采用酶联免疫吸附测定（ELISA）法检测血浆VEGF水平。

结果

HSPN组CC基因型分布（32%）和C等位基因频率（56%）显著高于对照组（分别为10%和35%）和非肾炎性过敏性紫癜组（分别为10%和33%）（P<0.01）。CC基因型的过敏性紫癜患儿肾炎发病率显著高于GG基因型患儿（76%比31%；P<0.01）。CC基因型患者的血浆VEGF水平（180.5±40.7 pg/mL）显著高于CG基因型（145.2±48.3 pg/mL）和GG基因型（101.5±26.5 pg/mL）患者（P<0.05）。