Ding Gui-Xia, Wang Chen-Hu, Che Ruo-Chen, Guan Wan-Zhen, Yuan Yang-Gang, Su Min, Zhang Ai-Hua, Huang Song-Ming
Department of Nephrology, Nanjing Children's Hospital, Nanjing Medical University, Nanjing, China.
World J Pediatr. 2016 Feb;12(1):49-54. doi: 10.1007/s12519-015-0048-9. Epub 2015 Nov 7.
Henoch-Schönlein purpura (HSP) or IgA-associated vasculitis is related to immune disturbances. Polymorphisms of the heat shock protein 70-2 gene (HSP70-2) and the tumor necrosis factor-a gene (TNF-α) are known to be associated with immune diseases. The purpose of this study was to investigate the likely association of HSP70-2 (+1267A/G) and TNF-α (+308A/G) gene polymorphisms with HSP in children.
The polymerase chain reaction restriction fragment length polymorphism method was used to detect the HSP70-2 and TNF-α polymorphisms in 205 cases of children with HSP and 53 controls; and the association of these polymorphisms with HSP and HSP nephritis (HSPN) was analyzed.
The G/G genotypic frequencies at the +1267A/G position of HSP70-2 in the HSP group (22.9%) were significantly higher than those in the healthy control group (9.4%) (χ(2)=4.764, P<0.05). The frequencies of the A/A, A/G and G/G genotypes of HSP70-2 in patients in the nephritis-free group and the HSPN group showed no statistically significant difference. The A/A genotype frequency at the +308G/A position of TNF-α in the HSP group was 8.3%, which was higher than that in the control group (χ(2)=6.447, P<0.05). The A allele frequency of TNF-α in the HSP group was higher than that in the control group, with a statistically significant difference (χ(2)=7.241, P<0.05).
The HSP70-2 (+1267A/G) and TNF-α (+308G/A) gene polymorphisms were associated with HSP in children. The G/G homozygosity of HSP70-2 and the A/A homozygosity of TNF-α may be genetic predisposing factors for HSP.
过敏性紫癜（HSP）或IgA相关性血管炎与免疫紊乱有关。已知热休克蛋白70 - 2基因（HSP70 - 2）和肿瘤坏死因子 - α基因（TNF - α）的多态性与免疫疾病相关。本研究的目的是探讨HSP70 - 2（+1267A/G）和TNF - α（+308A/G）基因多态性与儿童HSP的可能关联。
采用聚合酶链反应 - 限制性片段长度多态性方法检测205例HSP患儿和53例对照的HSP70 - 2和TNF - α多态性；并分析这些多态性与HSP及HSP肾炎（HSPN）的关联。
HSP组中HSP70 - 2基因+1267A/G位点的G/G基因型频率（22.9%）显著高于健康对照组（9.4%）（χ(2)=4.764，P<0.05）。无肾炎组和HSPN组患者中HSP70 - 2基因的A/A、A/G和G/G基因型频率无统计学差异。HSP组中TNF - α基因+308G/A位点的A/A基因型频率为8.3%，高于对照组（χ(2)=6.447，P<0.05）。HSP组中TNF - α基因的A等位基因频率高于对照组，差异有统计学意义（χ(2)=7.241，P<0.05）。
HSP70 - 2（+1267A/G）和TNF - α（+308G/A）基因多态性与儿童HSP相关。HSP70 - 2基因的G/G纯合性和TNF - α基因的A/A纯合性可能是HSP的遗传易感因素。
