Institute of Neurosciences, the Fourth Military Medical University, 17 Chang Le Xi Road, Xi'an, Shaanxi 710032, China.
Neurosci Lett. 2009 Nov 6;465(1):27-30. doi: 10.1016/j.neulet.2009.06.059. Epub 2009 Jun 24.
GFAP has long been adopted as the specific marker for pituicyte, a special type of astrocyte. GFAP and S100beta are two commonly used astrocyte markers. Their immunoreactivities differ in different regions of the brain. To our knowledge this issue has not been studied in pituicyte. In our preliminary study, we found that antibodies against GFAP and S100beta stained the pituicytes differently. A detailed investigation with both light and electron microscopic techniques was thus conducted in the rat. At light microscopic level, anti-GFAP and anti-S100beta stained 66.78% and 86.78% of the pituicytes, respectively. It was found at ultrastructural level that this difference was cell type specific. The parenchymatous pituicytes could be stained with antibodies against both GFAP and S100beta, whereas the fibrous pituicytes were only S100beta-immunoreactive. The functional significance of this cell type specificity remains to be elucidated.
胶质纤维酸性蛋白（GFAP）长期以来被用作垂体细胞的特异性标志物，垂体细胞是一种特殊类型的星形胶质细胞。GFAP 和 S100β是两种常用的星形胶质细胞标志物。它们在大脑的不同区域的免疫反应不同。据我们所知，这个问题在垂体细胞中尚未得到研究。在我们的初步研究中，我们发现针对 GFAP 和 S100β的抗体对垂体细胞的染色不同。因此，我们在大鼠中使用光镜和电镜技术进行了详细的研究。在光镜水平上，抗 GFAP 和抗 S100β分别染色了 66.78%和 86.78%的垂体细胞。在超微结构水平上发现，这种差异是细胞类型特异性的。实质垂体细胞可以被针对 GFAP 和 S100β的抗体染色，而纤维垂体细胞仅对 S100β呈免疫反应性。这种细胞类型特异性的功能意义仍有待阐明。
