提高接受定时人工授精的婆罗门牛青春期后小母牛和非泌乳母牛繁殖力的策略。
Strategies to improve fertility in Bos indicus postpubertal heifers and nonlactating cows submitted to fixed-time artificial insemination.
作者信息
Peres R F G, Claro I, Sá Filho O G, Nogueira G P, Vasconcelos J L M
机构信息
Departamento de Produção Animal, Faculdade de Medicina Veterinária e Zootecnia-UNESP, Botucatu SP 18618-000, Brazil.
出版信息
Theriogenology. 2009 Sep 15;72(5):681-9. doi: 10.1016/j.theriogenology.2009.04.026. Epub 2009 Jun 25.
Two experiments were designed to evaluate strategies to increase fertility of Bos indicus postpubertal heifers and nonlactating cows submitted to a fixed-time artificial insemination (TAI) protocol consisting of an intravaginal device containing 1.9 g of progesterone (CIDR) insertion+estradiol benzoate on Day 0, CIDR withdrawal+estradiol cypionate on Day 9, and TAI on Day 11. In Experiment 1, heifers (n=1153) received a new or an 18-d previously used CIDR and, on Day 9, prostaglandin F(2 alpha) (PGF(2 alpha))+0, 200, or 300 IU equine chorionic gonadotropin (eCG). Heifers treated with a new CIDR had greater (least squares means+/-SEM) serum concentration of progesterone on Day 9 (3.06+/-0.09 ng/mL vs. 2.53+/-0.09 ng/mL; P<0.05) and a smaller follicle at TAI (11.61+/-0.11 mm vs. 12.05+/-0.12 mm; P<0.05). Heifers with smaller follicles at TAI had lesser serum progesterone concentrations on Day 18 and reduced rates of ovulation, conception, and pregnancy (P<0.05). Treatment with eCG improved (P<0.05) follicle diameter at TAI (11.50+/-0.10mm, 11.90+/-0.11 mm, and 12.00+/-0.10mm for 0, 100, and 200 IU, respectively), serum progesterone concentration on Day 18 (2.77+/-0.11 ng/mL, 3.81+/-0.11 ng/mL, and 4.87+/-0.11 ng/mL), and rates of ovulation (83.8%, 88.5%, and 94.3%) and pregnancy (41.3%, 47.0%, and 46.7%). In Experiment 2, nonlactating Nelore cows (n=702) received PGF(2 alpha) treatment on Days 7 or 9 and, on Day 9, 0 or 300 IU eCG. Cows receiving PGF(2 alpha) on Day 7 had lesser serum progesterone concentrations on Day 9 (3.05+/-0.21 ng/mL vs. 4.58+/-0.21 ng/mL; P<0.05), a larger follicle at TAI (11.54+/-0.21 mm vs. 10.84+/-0.21 mm; P<0.05), and improved (P<0.05) rates of ovulation (85.4% vs. 77.0%), conception (60.9% vs. 47.2%), and pregnancy (52.0% vs. 36.4%). Treatment with eCG improved (P<0.05) serum progesterone concentration on Day 18 (3.24+/-0.14 ng/mL vs. 4.55+/-0.14 ng/mL) and the rates of ovulation (72.4% vs. 90.0%) and pregnancy (37.5% vs. 50.8%). In conclusion, giving PGF(2 alpha) earlier in the protocol in nonlactating cows and eCG treatment in postpubertal heifers and nonlactating cows improved fertility in response to a TAI (progesterone+estradiol) protocol.
设计了两个试验来评估提高印度瘤牛青春期后小母牛和非泌乳母牛繁殖力的策略，这些牛采用了固定时间人工授精（TAI）方案，该方案包括在第0天插入含1.9 g孕酮的阴道内装置（CIDR）+苯甲酸雌二醇，在第9天取出CIDR+环丙孕酮，以及在第11天进行TAI。在试验1中，小母牛（n = 1153）接受新的或之前使用过18天的CIDR，并在第9天接受前列腺素F（2α）（PGF（2α））+0、200或300 IU马绒毛膜促性腺激素（eCG）。用新CIDR处理的小母牛在第9天的血清孕酮浓度更高（最小二乘均值±标准误）（3.06±0.09 ng/mL对2.53±0.09 ng/mL；P<0.05），且在TAI时卵泡较小（11.61±0.11 mm对12.05±0.12 mm；P<0.05）。在TAI时卵泡较小的小母牛在第18天的血清孕酮浓度较低，排卵、受孕和妊娠率降低（P<0.05）。用eCG处理可改善（P<0.05）TAI时的卵泡直径（0、100和200 IU时分别为11.50±0.10mm、11.90±0.11 mm和12.00±0.10mm）、第18天的血清孕酮浓度（2.77±0.11 ng/mL、3.81±0.11 ng/mL和4.87±0.11 ng/mL）以及排卵率（83.8%、88.5%和94.3%）和妊娠率（41.3%、47.0%和46.7%）。在试验2中，非泌乳内洛尔牛（n = 702）在第7天或第9天接受PGF（2α）处理，并在第9天接受0或300 IU eCG。在第7天接受PGF（2α）处理的母牛在第9天的血清孕酮浓度较低（3.05±0.21 ng/mL对4.58±0.21 ng/mL；P<0.05），在TAI时卵泡较大（11.54±0.21 mm对10.84±0.21 mm；P<0.05），且排卵率（85.4%对77.0%）、受孕率（60.9%对47.2%）和妊娠率（52.0%对36.4%）提高（P<0.05）。用eCG处理可改善（P<0.05）第18天的血清孕酮浓度（3.24±0.14 ng/mL对4.55±0.14 ng/mL）以及排卵率（72.4%对90.0%）和妊娠率（37.5%对50.8%）。总之，在非泌乳母牛的方案中更早给予PGF（2α）以及在青春期后小母牛和非泌乳母牛中进行eCG处理可提高对TAI（孕酮+雌二醇）方案的繁殖力。
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