Sá Filho Manoel Francisco de, Gonella-Diaza Angela Maria, Sponchiado Mariana, Mendanha Marcio Ferreira, Pugliesi Guilherme, Ramos Roney Dos Santos, Andrade Sónia Cristina da Silva, Gasparin Gustavo, Coutinho Luiz Lehmann, Goissis Marcelo Demarchi, Mesquita Fernando Silveira, Baruselli Pietro Sampaio, Binelli Mario
Departamento de Reprodução Animal, FMVZ-USP, São Paulo, SP Brazil.
Departamento de Genética e Biologia Evolutiva, IB-USP-, São Paulo, SP Brazil.
J Anim Sci Biotechnol. 2017 Nov 1;8:79. doi: 10.1186/s40104-017-0211-3. eCollection 2017.
This study evaluated the impact of hormonal modulation at the onset of proestrus on ovarian response and uterine gene expression of beef cows.
A total of 172 anestrous beef cows were assigned to one of four groups according to the treatment with estradiol cypionate (ECP) and/or equine chorionic gonadotropin (eCG) [CON ( = 43), ECP (n = 43), eCG ( = 44) and ECP + eCG ( = 42)].
ECP-treated cows (ECP and ECP + eCG groups) presented greater occurrence of estrus (44.6% vs. 65.4%; = 0.01) and pregnancy per AI [47.1% vs. 33.3%; = 0.07], but similar progesterone (P4) concentration at subsequent diestrus than cows not treated with ECP (CON and eCG groups). Nonetheless, eCG-treated cows (eCG and ECP + eCG groups) presented larger follicle at timed AI (12.6 ± 0.3 vs. 13.5 ± 0.3 mm; = 0.03), greater ovulation rate (96.5% vs. 82.6%; = 0.008) and greater P4 concentration at d 6 (3.9 ± 0.2 vs. 4.8 ± 0.2 ng/mL; = 0.001) than cows not treated with eCG (CON and ECP groups). Next, cows with a new corpus luteum 6 d after TAI were submitted to uterine biopsy procedure. Uterine fragments [CON ( = 6), ECP (n = 6)] were analyzed by RNA-Seq and a total of 135 transcripts were differentially expressed between groups (73 genes up-regulated by ECP treatment). Subsequently, uterine samples were analyzed by qPCR (genes associated with cell proliferation). ECP treatment induced greater abundance of ( = 0.07) and ( = 0.02), whereas suppressed ( = 0.09) expression. Conversely, eCG treatment increased abundance of ( = 0.06), ( = 0.09), and ( = 0.05), whereas it reduced transcription of ( = 0.05). Collectively, supplementation with ECP or eCG at the onset of proestrous of anestrous beef cows influenced ovarian responses, global and specific endometrial gene expression.
Proestrus estradiol regulate the endometrial transcriptome, particularly stimulating proliferative activity in the endometrium.
本研究评估了发情前期激素调节对肉牛卵巢反应和子宫基因表达的影响。
根据环丙孕酮（ECP）和/或马绒毛膜促性腺激素（eCG）的处理，将172头处于乏情期的肉牛分为四组之一[对照组（n = 43）、ECP组（n = 43）、eCG组（n = 44）和ECP + eCG组（n = 42）]。
接受ECP处理的母牛（ECP组和ECP + eCG组）发情发生率更高（44.6%对65.4%；P = 0.01），每次人工授精后的妊娠率也更高[47.1%对33.3%；P = 0.07]，但与未接受ECP处理的母牛（对照组和eCG组）相比，在随后的间情期孕酮（P4）浓度相似。尽管如此，接受eCG处理的母牛（eCG组和ECP + eCG组）在定时人工授精时卵泡更大（12.6±0.3对13.5±0.3毫米；P = 0.03），排卵率更高（96.5%对82.6%；P = 0.008），在第6天P4浓度更高（3.9±0.2对4.8±0.2纳克/毫升；P = 0.001），高于未接受eCG处理的母牛（对照组和ECP组）。接下来，在定时人工授精后6天有新黄体的母牛接受子宫活检程序。通过RNA测序分析子宫组织[对照组（n = 6）、ECP组（n = 6）]，两组之间共有135个转录本差异表达（73个基因经ECP处理后上调）。随后，通过qPCR分析子宫样本（与细胞增殖相关的基因）。ECP处理诱导了更大丰度的[基因名称1]（P = 0.07）和[基因名称2]（P = 0.02），而抑制了[基因名称3]（P = 0.09）的表达。相反，eCG处理增加了[基因名称4]（P = 0.06）、[基因名称5]（P = 0.09）和[基因名称6]（P = 0.05）的丰度，而降低了[基因名称7]（P = 0.05）的转录。总体而言，在乏情期肉牛发情前期补充ECP或eCG会影响卵巢反应、整体和特定的子宫内膜基因表达。
发情前期雌二醇调节子宫内膜转录组，特别是刺激子宫内膜的增殖活性。
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