Bornet E P, Entman M L, Van Winkle W B, Schwartz A, Lehotay D C, Levey G S
Biochim Biophys Acta. 1977 Jul 14;468(2):188-93. doi: 10.1016/0005-2736(77)90113-4.
The role of cyclic 3',5'-AMP in modulating sarcoplasmic reticulum from fast skeletal muscle was studied. The rate of Ca2+ uptake was stimulated in the presence of protein kinase plus 1 micron cyclic AMP. The stimulation was absent when denatured protein kinase was used. When an adenylate cyclase inhibitor was added, the uptake rates fell to 55% of control. This decrease in rate was partially overcome by 1 micron cyclic AMP. A modulating role for cyclic AMP in fast skeletal muscle is proposed.
研究了环3',5'-腺苷酸在调节快收缩骨骼肌肌浆网中的作用。在蛋白激酶加1微摩尔环腺苷酸存在的情况下，钙离子摄取速率受到刺激。使用变性蛋白激酶时则无此刺激作用。添加腺苷酸环化酶抑制剂后，摄取速率降至对照的55%。1微摩尔环腺苷酸可部分克服这种速率降低。提出了环腺苷酸在快收缩骨骼肌中的调节作用。
