慢性荨麻疹患者中C5a诱导的体外嗜碱性粒细胞活化：一项初步研究。
C5a-induced in vitro basophil activation in patients with chronic urticaria: a pilot study.
作者信息
Korosec Peter, Subic Tjasa, Adamic Katja, Silar Mira, Kosnik Mitja
机构信息
University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia.
出版信息
Wien Klin Wochenschr. 2009;121(9-10):339-43. doi: 10.1007/s00508-009-1168-9.
BACKGROUND
Complement component 5a (C5a) might be involved in the formation of wheals in patients with chronic urticaria (CU). We sought to compare the in vitro responsiveness of basophils to C5a in patients with CU and in a control group.
METHODS
Basophil surface expression of activation marker CD63 induced by C5a, anti-FcepsilonRI mAb or anti-IgE pAb was measured using flow cytometry in 17 patients with CU and in 10 healthy controls.
RESULTS
Patients with CU showed significantly greater basophil CD63 surface expression induced by C5a (median [interquartile range]; 16.4% [13-25.1]; P = 0.011) than the group of healthy controls (10.7% [7.2-16.8]). In contrast, basophil CD63 response to anti-IgE and anti-FcepsilonRI was lower in the CU group (12.3% [6-36.3]; 25.9% [12.5-60.5]) than in the control group (51.7% [6.7-84.3]; 62.1% [9.7-89.2]), although not statistically significant.
CONCLUSION
Results of this pilot study suggest that patients with CU might have an enhanced basophil response to stimulation with C5a, indicating that further studies in CU basophil responsiveness are needed.
背景
补体成分5a（C5a）可能参与慢性荨麻疹（CU）患者风团的形成。我们试图比较CU患者和对照组中嗜碱性粒细胞对C5a的体外反应性。
方法
采用流式细胞术检测17例CU患者和10例健康对照者中，C5a、抗FcepsilonRI单克隆抗体或抗IgE多克隆抗体诱导的嗜碱性粒细胞表面活化标志物CD63的表达。
结果
CU患者中，C5a诱导的嗜碱性粒细胞CD63表面表达（中位数[四分位间距]；16.4%[13 - 25.1]；P = 0.011）显著高于健康对照组（10.7%[7.2 - 16.8]）。相比之下，CU组中嗜碱性粒细胞对抗IgE和抗FcepsilonRI的CD63反应（12.3%[6 - 36.3]；25.9%[12.5 - 60.5]）低于对照组（51.7%[6.7 - 84.3]；62.1%[9.7 - 89.2]），尽管差异无统计学意义。
结论
这项初步研究结果表明，CU患者的嗜碱性粒细胞对C5a刺激的反应可能增强，提示需要对CU患者嗜碱性粒细胞反应性进行进一步研究。
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