Saito M
Biochim Biophys Acta. 1977 Jul 21;498(1):316-24. doi: 10.1016/0304-4165(77)90269-0.
Effect of adenosine on the level of guanosine 3',5'-monophosphate in guinea pig cerebellar slices was investigated. Adenosine increased the concentration of guanosine 3',5'-monophosphate in the slices 3--4 fold. Upon removal of adenosine from the medium, the concentration of guanosine 3',5'-monophosphate returned to the initial level. AMP, ADP or ATP also increased the guanosine 3',5'-monophosphate level to the same extent as adenosine, while adenine or other nucleosides were not effective. In the absence of Ca2+ in the incubation medium, adenosine did not increase the concentration of guanosine 3',5'-monophosphate in cerebellar slices although level of adenosine 3',5'-monophosphate was elevated by adenosine. Anticholinergic agents, adrenergic blocking agents or antihistaminics did not prevent the increase of guanosine 3',5'-monophosphate by adenosine indicating that the effect of adenosine was not mediated by the release of neurotransmitters. The combination of adenosine with depolarizing agents showed an additive effect on the level of guanosine 3',5'-monophosphate indicating that adenosine increased the level of guanosine 3',5'-monophosphate by a different mechanism from the depolarization.
研究了腺苷对豚鼠小脑切片中3',5'-环磷酸鸟苷水平的影响。腺苷使切片中3',5'-环磷酸鸟苷的浓度增加了3至4倍。从培养基中去除腺苷后，3',5'-环磷酸鸟苷的浓度恢复到初始水平。AMP、ADP或ATP也能使3',5'-环磷酸鸟苷水平增加到与腺苷相同的程度，而腺嘌呤或其他核苷则无效。在孵育培养基中不存在Ca2+的情况下，腺苷不会增加小脑切片中3',5'-环磷酸鸟苷的浓度，尽管腺苷会提高3',5'-环磷酸腺苷的水平。抗胆碱能药物、肾上腺素能阻断剂或抗组胺药不会阻止腺苷引起的3',5'-环磷酸鸟苷增加，这表明腺苷的作用不是由神经递质释放介导的。腺苷与去极化剂联合使用对3',5'-环磷酸鸟苷水平显示出相加作用，这表明腺苷增加3',5'-环磷酸鸟苷水平的机制与去极化不同。
