Iizuka H, Adachi K, Halprin K M, Levine V
Biochim Biophys Acta. 1976 Oct 22;444(3):685-93. doi: 10.1016/0304-4165(76)90315-9.
Adenosine, AMP, ADP and ATP activated adenylate cyclase in pig skin (epidermis) slices resulting in the accumulation of cyclic AMP. This effect was highly potentiated by the addition of the cyclic AMP-phosphodiesterase inhibitor, papaverine. But another inhibitor, theophylline, strongly blocked the activation of adenylate cyclase by adenosine and adenine nucleotides. Theophylline apparently competed with adenosine for the cell surface receptor. Like theophylline, the addition of adenine alone caused no accumulation of cyclic AMP, but it significantly inhibited the stimulatory effect of adenosine. Guanosine, or guanine, cytidine, uridine, or thymidine nucleotides had no effect on the accumulation of cyclic AMP. Among other adenine nucleotides we tested, adenosine 5'-monophosphoramidate, but not adenosine 5'-monosulfate significantly increased cyclic AMP especially with the addition of papaverine. Neither 2'- nor 3'-adenylic acid were effective. Our data indicate that pig epidermis has four specific and independent adenylate cyclase systems for adenosine (and adenine nucleotides), histamine, epinephrine and prostaglandin E.
腺苷、AMP、ADP和ATP可激活猪皮肤（表皮）切片中的腺苷酸环化酶，导致环磷酸腺苷（cAMP）的积累。加入环磷酸腺苷磷酸二酯酶抑制剂罂粟碱后，这种效应得到了极大的增强。但另一种抑制剂茶碱强烈阻断了腺苷和腺嘌呤核苷酸对腺苷酸环化酶的激活作用。茶碱显然与腺苷竞争细胞表面受体。与茶碱一样，单独加入腺嘌呤不会导致cAMP的积累，但它显著抑制了腺苷的刺激作用。鸟苷、鸟嘌呤、胞苷、尿苷或胸苷核苷酸对cAMP的积累没有影响。在我们测试的其他腺嘌呤核苷酸中，5'-单磷酸腺苷酰胺（而非5'-单硫酸腺苷）尤其在加入罂粟碱后显著增加了cAMP。2'-腺苷酸和3'-腺苷酸均无效。我们的数据表明，猪表皮具有四个针对腺苷（和腺嘌呤核苷酸）、组胺、肾上腺素和前列腺素E的特异性且独立的腺苷酸环化酶系统。
