Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Crit Rev Microbiol. 2009;35(3):197-220. doi: 10.1080/10408410902983129.
Recombination plays a significant role in bacteriophage biology. Functions promoting recombination are involved in key stages of phage multiplication and drive phage evolution. Their biological role is reflected by the great variety of phages existing in the environment. This work presents the role of recombination in the phage life cycle and highlights the discrete character of phage-encoded recombination functions (anti-RecBCD activities, 5' --> 3' DNA exonucleases, single-stranded DNA binding proteins, single-stranded DNA annealing proteins, and recombinases). The focus of this review is on phage proteins that initiate genetic exchange. Importance of recombination is reviewed based on the accepted coli-phages T4 and lambda models, the recombination system of phage P22, and the recently characterized recombination functions of Bacillus subtilis phage SPP1 and mycobacteriophage Che9c. Key steps of the molecular mechanisms involving phage recombination functions and their application in molecular engineering are discussed.
重组在噬菌体生物学中起着重要作用。促进重组的功能涉及噬菌体繁殖的关键阶段，并推动噬菌体进化。它们的生物学作用反映在环境中存在的大量噬菌体中。这项工作介绍了重组在噬菌体生命周期中的作用，并强调了噬菌体编码的重组功能（抗 RecBCD 活性、5'-->3'DNA 外切酶、单链 DNA 结合蛋白、单链 DNA 退火蛋白和重组酶）的离散特征。本综述的重点是启动遗传交换的噬菌体蛋白。基于公认的大肠杆菌噬菌体 T4 和 lambda 模型、噬菌体 P22 的重组系统以及最近表征的枯草芽孢杆菌噬菌体 SPP1 和分枝杆菌噬菌体 Che9c 的重组功能，综述了重组的重要性。讨论了涉及噬菌体重组功能的分子机制的关键步骤及其在分子工程中的应用。
