Jiang H, Salinas F, Kodadek T
Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA.
Biochem Biophys Res Commun. 1997 Feb 24;231(3):600-5. doi: 10.1006/bbrc.1997.6160.
A central reaction in homologous recombination is synapsis, which involves invasion of duplex DNA by a homologous single strand. A key intermediate in this process is the presynaptic filament, a protein-DNA complex composed of a "strand transferase" polymerized along the invading single strand. In this report, the organization and mechanism of assembly of the bacteriophage T4 presynaptic filament are explored. Three T4 proteins, encoded by the uvsX, uvsY and 32 genes, are involved in this process. It is demonstrated that a well-defined series of events involving multiple protein-DNA and protein-protein interactions is required to mediate a transition from an initial gene 32-DNA complex to a mature presynaptic filament in which the UvsX and UvsY proteins are in contact with the DNA and each other, while most or all of the gene 32 protein is removed from the complex.
同源重组中的一个核心反应是联会，它涉及同源单链对双链DNA的侵入。这一过程中的一个关键中间体是突触前细丝，它是一种蛋白质-DNA复合物，由沿着侵入单链聚合的“链转移酶”组成。在本报告中，对噬菌体T4突触前细丝的组装组织和机制进行了探索。uvsX、uvsY和32基因编码的三种T4蛋白参与了这一过程。结果表明，需要一系列明确的事件，包括多种蛋白质-DNA和蛋白质-蛋白质相互作用，来介导从最初的基因32-DNA复合物到成熟突触前细丝的转变，在成熟的突触前细丝中，UvsX和UvsY蛋白与DNA以及彼此相互接触，而大部分或所有的基因32蛋白则从复合物中去除。
