人类通用转录因子TFIIE大亚基中的结构基序和潜在的sigma同源性

Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE.

作者信息

Ohkuma Y, Sumimoto H, Hoffmann A, Shimasaki S, Horikoshi M, Roeder R G

机构信息

Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.

出版信息

Nature. 1991 Dec 5;354(6352):398-401. doi: 10.1038/354398a0.

DOI:10.1038/354398a0

PMID:1956403

Abstract

The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. Here we report the cloning and structure of a complementary DNA encoding a functional human TFIIE-alpha. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. The sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation.

摘要

通用转录因子TFIIE与RNA聚合酶II及其他通用因子一起，在真核生物转录起始过程中发挥着重要作用。人TFIIE由相对分子质量为57,000的两个亚基（TFIIE-α）和34,000的亚基（TFIIE-β）组成，在RNA聚合酶II和TFIIF之后加入起始前复合物。在此，我们报道了编码功能性人TFIIE-α的互补DNA的克隆及结构。TFIIE-α与TFIIE-β一起是转录起始所必需的，并且重组TFIIE-α在体外转录试验中可以完全替代天然亚基。该序列包含几个有趣的结构基序（亮氨酸重复序列、锌指结构和螺旋-转角-螺旋结构），以及与细菌σ因子的序列相似性，这表明其直接参与转录起始的调控。