Toai Tran Cong, Thao Huynh Duy, Thao Nguyen Phuong, Gargiulo Ciro, Ngoc Phan Kim, Van Pham Hung, Strong D Michael
Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam.
Cell Tissue Bank. 2010 Aug;11(3):269-80. doi: 10.1007/s10561-009-9141-4. Epub 2009 Jun 30.
It is well accepted that human umbilical cord blood (UCB) is a source of mesenchymal stem cells (MSCs) which are able to differentiate into different cell phenotypes such as osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes and neurons. The aim of this study was to isolate MSCs from human UCB to determine their osteogenic potential by using different kinds of osteogenic medium. Eventually, only those MSCs cultured in osteogenic media enriched with vitamin D(2) and FGF9, were positive for osteocalcin by RT-PCR. All these cells were positive for alizarin red, alkaline phosphatase and Von Kossa. The results obtained from RT-PCR have confirmed that osteogenesis is complete by expression of the osteocalcin marker. In conclusion, vitamin D(2), at least in vitro, may replace vitamin D(3) as an osteogenic stimulator factor for MSC differentiation.
人们普遍认为，人脐带血（UCB）是间充质干细胞（MSC）的一个来源，这些间充质干细胞能够分化为不同的细胞表型，如成骨细胞、软骨细胞、脂肪细胞、肌细胞、心肌细胞和神经元。本研究的目的是从人脐带血中分离间充质干细胞，通过使用不同种类的成骨培养基来确定它们的成骨潜力。最终，只有那些在富含维生素D(2)和FGF9的成骨培养基中培养的间充质干细胞，通过RT-PCR检测骨钙素呈阳性。所有这些细胞茜素红、碱性磷酸酶和冯·科萨染色均呈阳性。RT-PCR获得的结果证实，通过骨钙素标志物的表达，成骨过程已完成。总之，至少在体外，维生素D(2)可能替代维生素D(3)作为间充质干细胞分化的成骨刺激因子。
