Yamao F, Andachi Y, Muto A, Ikemura T, Osawa S
Department of Biology, Faculty of Science, Nagoya University, Japan.
Nucleic Acids Res. 1991 Nov 25;19(22):6119-22. doi: 10.1093/nar/19.22.6119.
Transfer RNAs of Mycoplasma capricolum were separated by two-dimensional polyacrylamide gel electrophoresis, and the relative abundance of each of the 28 known tRNA species was measured. There existed a correlation between the relative amount of isoacceptor tRNAs and the frequency in choosing synonymous codons that could be translated by the isoacceptors. Furthermore, it was observed that the total amount of tRNAs for a particular amino acid was paralleled by the composition of the amino acid in ribosomal proteins. A similar relationship was obtained from reexamination of the previous data on Escherichia coli tRNAs, suggesting that the amount of tRNAs for an amino acid is affected by the usage of the amino acid in proteins.
通过二维聚丙烯酰胺凝胶电泳分离了山羊支原体的转运RNA，并测定了28种已知tRNA种类中每一种的相对丰度。同功受体tRNA的相对量与可由这些同功受体翻译的同义密码子的选择频率之间存在相关性。此外，观察到特定氨基酸的tRNA总量与核糖体蛋白质中该氨基酸的组成平行。对大肠杆菌tRNA的先前数据重新检查也得到了类似的关系，这表明氨基酸的tRNA量受蛋白质中该氨基酸使用情况的影响。
