Doherty A J, Worrall A F, Connolly B A
Department of Biochemistry (SERC Molecular recognition Initiative Centre), University of Southampton, UK.
Nucleic Acids Res. 1991 Nov 25;19(22):6129-32. doi: 10.1093/nar/19.22.6129.
The sequence selectivity of DNase 1 cleavage has been investigated by site-directed mutagenesis of a chemically synthesised gene. Two key DNA binding residues have been conservatively altered (Y76F and R41K) or have had their side-chains truncated (Y76A and R41A) and the effect on the cleavage of tyr T promoter DNA has been noted. It would appear from these studies that DNase 1 is not sensitive to minor groove width via these DNA-contacting residues, and it is suggested that DNA helical stiffness is a controlling parameter in determining DNase 1 sequence selectivity.
通过对化学合成基因进行定点诱变，研究了DNase 1切割的序列选择性。两个关键的DNA结合残基被保守性改变（Y76F和R41K）或其侧链被截短（Y76A和R41A），并记录了对酪氨酸T启动子DNA切割的影响。从这些研究中可以看出，DNase 1通过这些与DNA接触的残基对小沟宽度不敏感，并且有人提出DNA螺旋刚度是决定DNase 1序列选择性的控制参数。
