Lang N P, Kadlubar F F
Dept. of Surgery, School of Medicine, University of Ark. for Med. Sci., Little Rock.
Prog Clin Biol Res. 1991;372:33-47.
Our method to evaluate acetylator phenotype and N-oxidation phenotype with a single caffeine dose and a single urine specimen collection has enabled us to examine acetylation and N-oxidation by two enzyme systems that have a known genetic polymorphism and are important in aromatic amine metabolism. Based on available data, we have hypothesized patients with urinary bladder cancer will have a higher frequency of rapid N-oxidation and slow acetylation phenotypes when compared to controls. On the other hand, patients with colorectal cancer should contain a higher proportion of both rapid N-oxidation phenotype and rapid acetylation phenotype when compared to the control group. In contrast, lung cancer patients, should contain an increased frequency of rapid arylamine N-oxidation phenotypes with the frequency of acetyltransferase phenotype being greater or perhaps the same as in the control group.
我们采用单次服用咖啡因剂量并单次收集尿液样本的方法来评估乙酰化表型和N-氧化表型，这使我们能够通过两个具有已知基因多态性且在芳香胺代谢中起重要作用的酶系统来检测乙酰化和N-氧化。根据现有数据，我们推测与对照组相比，膀胱癌患者快速N-氧化和慢乙酰化表型的频率会更高。另一方面，与对照组相比，结直肠癌患者快速N-氧化表型和快速乙酰化表型的比例应该更高。相比之下，肺癌患者快速芳胺N-氧化表型的频率应增加，乙酰转移酶表型的频率与对照组相比更大或可能相同。
