成体单能生殖系干细胞多能性的诱导。

Induction of pluripotency in adult unipotent germline stem cells.

作者信息

Ko Kinarm, Tapia Natalia, Wu Guangming, Kim Jeong Beom, Bravo Marcos J Araúzo, Sasse Philipp, Glaser Tamara, Ruau David, Han Dong Wook, Greber Boris, Hausdörfer Kirsten, Sebastiano Vittorio, Stehling Martin, Fleischmann Bernd K, Brüstle Oliver, Zenke Martin, Schöler Hans R

机构信息

Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster 48149, Germany.

出版信息

Cell Stem Cell. 2009 Jul 2;5(1):87-96. doi: 10.1016/j.stem.2009.05.025.

DOI:10.1016/j.stem.2009.05.025

PMID:19570517

Abstract

Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.

摘要

具有与胚胎干细胞相似特征的小鼠和人类干细胞已从睾丸细胞中获得。尽管已从小鼠新生睾丸的特定生殖系干细胞（GSCs）中获得了多能干细胞，但迄今为止，仅从小鼠成年睾丸的特定细胞中获得了多能干细胞。在本研究中，我们描述了一种从成年单能GSCs中获得生殖系来源的多能干细胞（gPS细胞）的可靠且可重复的方案。通过体外和体内分化，包括生殖细胞贡献和传递，证实了gPS细胞的多能性。通过克隆分析确定，gPS细胞确实起源于单能GSCs。我们提出，转化过程需要一个GSC培养微环境，该微环境取决于接种的GSCs的初始数量和培养时间的长短。