Paik Man-Jeong, Kim Hoon, Lee Jinwoo, Brand Jerry, Kim Kyoung-Rae
Metabolomic Analysis Laboratory, Institute for Neuroregeneration and Stem Cell Research, and Department of Molecular Science and Technology, School of Medicine, Ajou University, Suwon 443-721, South Korea.
J Chromatogr A. 2009 Jul 31;1216(31):5917-23. doi: 10.1016/j.chroma.2009.06.051. Epub 2009 Jun 21.
Microalgal lipids were separated into two fractions, triacylglycerols (TAGs) and free fatty acids (FFAs), by solid-phase extraction employing sodium carbonate as the sorbent and dichloromethane (20% by volume) in n-hexane as the extracting solvent. The TAG fraction was then saponified, followed by acidification, extraction and tert-butyldimethylsilyl esterification. The FFA fraction was directly acidified, extracted and derivatized. From the lipid extracts of eight microalgal species examined, a total of 13 fatty acids were detected in the TAG fractions and nine were found in the FFA fractions, with at much higher total TAG content in all microalgae. Oleic acid was the most prominent fatty acid in three species, alpha-linolenic acid was more abundant in two others, and palmitic acid was present in highest concentration in the remaining three species.
微藻脂质通过固相萃取法被分离为两个部分，即三酰甘油（TAGs）和游离脂肪酸（FFAs），萃取过程使用碳酸钠作为吸附剂，以正己烷中体积分数为20%的二氯甲烷作为萃取溶剂。然后，对TAG部分进行皂化处理，接着进行酸化、萃取和叔丁基二甲基硅基酯化。FFA部分则直接进行酸化、萃取和衍生化。在所检测的8种微藻的脂质提取物中，TAG部分共检测到13种脂肪酸，FFA部分检测到9种脂肪酸，且所有微藻中的总TAG含量要高得多。油酸是三种微藻中最主要的脂肪酸，α-亚麻酸在另外两种微藻中含量更高，棕榈酸在其余三种微藻中浓度最高。
