Yang Dawei, Song Donghui, Kind Tobias, Ma Yan, Hoefkens Jens, Fiehn Oliver
Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, 67 Dong Chang Xi Lu, Liaocheng, Shandong, 252000, P. R. China.
Department of Marine Science, College of Marine Science & Engineering, Tianjin University of Science & Technology 29, the 13th St., TEDA, Tianjin, 300457, P. R. China.
PLoS One. 2015 Sep 16;10(9):e0137948. doi: 10.1371/journal.pone.0137948. eCollection 2015.
Chlamydomonas reinhardtii accumulates lipids under complete nutrient starvation conditions while overall growth in biomass stops. In order to better understand biochemical changes under nutrient deprivation that maintain production of algal biomass, we used a lipidomic assay for analyzing the temporal regulation of the composition of complex lipids in C. reinhardtii in response to nitrogen and sulfur deprivation. Using a chip-based nanoelectrospray direct infusion into an ion trap mass spectrometer, we measured a diversity of lipid species reported for C. reinhardtii, including PG phosphatidylglycerols, PI Phosphatidylinositols, MGDG monogalactosyldiacylglycerols, DGDG digalactosyldiacylglycerols, SQDG sulfoquinovosyldiacylglycerols, DGTS homoserine ether lipids and TAG triacylglycerols. Individual lipid species were annotated by matching mass precursors and MS/MS fragmentations to the in-house LipidBlast mass spectral database and MS2Analyzer. Multivariate statistics showed a clear impact on overall lipidomic phenotypes on both the temporal and the nutrition stress level. Homoserine-lipids were found up-regulated at late growth time points and higher cell density, while triacyclglycerols showed opposite regulation of unsaturated and saturated fatty acyl chains under nutritional deprivation.
莱茵衣藻在完全营养饥饿条件下积累脂质，而生物量的总体生长则停止。为了更好地理解营养剥夺条件下维持藻类生物量生产的生化变化，我们使用脂质组学分析方法来分析莱茵衣藻中复合脂质组成的时间调控，以响应氮和硫的剥夺。通过基于芯片的纳米电喷雾直接注入离子阱质谱仪，我们测量了莱茵衣藻中多种已报道的脂质种类，包括磷脂酰甘油（PG）、磷脂酰肌醇（PI）、单半乳糖基二酰基甘油（MGDG）、双半乳糖基二酰基甘油（DGDG）、磺基喹喔啉基二酰基甘油（SQDG）、高丝氨酸醚脂（DGTS）和三酰基甘油（TAG）。通过将质量前体和MS/MS碎片与内部脂质爆炸质谱数据库和MS2Analyzer进行匹配，对单个脂质种类进行注释。多变量统计显示，时间和营养应激水平对总体脂质组学表型都有明显影响。在生长后期和较高细胞密度时，发现高丝氨酸脂上调，而在营养剥夺条件下，三酰甘油中不饱和脂肪酸酰基链和饱和脂肪酸酰基链呈现相反的调控。
