Scherzer J, Fayrer-Hosken R A, Aceves M, Hurley D J, Ray L E, Jones L, Heusner G L
College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Aust Vet J. 2009 Jul;87(7):275-9. doi: 10.1111/j.1751-0813.2009.00452.x.
We evaluated combinations of two commercial semen extenders and three concentrations of glycerol to determine the combination that yielded the highest post-thaw sperm motility.
A randomised 2 x 3 block design was used.
Semen was collected from four stallions (6 collections per stallion). The sample was diluted with either a dried skim-milk glucose extender (EZ Mixin Original Formula) or a chemically defined, milk-free diluent (INRA 96), and each was used in combination with 2%, 3% or 4% glycerol in standard commercial freezing medium. Sperm motility was assessed by microscopy in fresh and post-thaw semen.
There was a significant difference between the two extenders in the motility of spermatozoa after cryopreservation (48.9% for INRA 96; 38.6% for EZ Mixin OF; P < 0.0001). Glycerol at 4% in freezing medium yielded the highest post-thaw motility, significantly better than 2% (P < 0.05). Three of four stallions had significantly higher post-thaw motility using INRA 96 relative to EZ Mixin OF (P < 0.01), and two of four stallions had significantly higher post-thaw motility using 4% glycerol (P < 0.05). The combination of INRA 96 and 4% glycerol in freezing medium gave the highest average post-thaw motility of 51.5%.
In this study, INRA 96 combined with 4% glycerol yielded an average recovery of progressively motile sperm consistently above the 35% target.
我们评估了两种商用精液稀释剂与三种甘油浓度的组合，以确定解冻后精子活力最高的组合。
采用随机2×3区组设计。
从四匹种马采集精液（每匹种马采集6次）。样本用干燥脱脂乳葡萄糖稀释剂（EZ Mixin原装配方）或化学成分明确的无乳稀释剂（INRA 96）进行稀释，每种稀释剂均与标准商用冷冻培养基中的2%、3%或4%甘油联合使用。通过显微镜检查新鲜精液和解冻后精液的精子活力。
两种稀释剂在冷冻保存后精子的活力存在显著差异（INRA 96为48.9%；EZ Mixin OF为38.6%；P<0.0001）。冷冻培养基中4%的甘油产生最高的解冻后活力，显著优于2%（P<0.05）。四匹种马中有三匹使用INRA 96相对于EZ Mixin OF解冻后活力显著更高（P<0.01），四匹种马中有两匹使用4%甘油解冻后活力显著更高（P<0.05）。冷冻培养基中INRA 96与4%甘油的组合产生最高的平均解冻后活力，为51.5%。
在本研究中，INRA 96与4%甘油联合使用时，渐进性活动精子的平均回收率始终高于35%的目标。
