用于微阵列上SNP检测的金属离子螯合辅助连接（CHESS LIGA）

Metal ion CHElate-aSSisted LIGAtion (CHESS LIGA) for SNP detection on microarrays.

作者信息

Le Thi Hien, Oretskaya Tatiana S, Zatsepin Timofei S

机构信息

Chemistry Department, M.V. Lomonossov Moscow State University, Leninskije Gory, Moscow 119991, Russia.

出版信息

Bioorg Med Chem Lett. 2009 Aug 1;19(15):4018-21. doi: 10.1016/j.bmcl.2009.06.033. Epub 2009 Jun 13.

DOI:10.1016/j.bmcl.2009.06.033

PMID:19574044

Abstract

We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.

摘要

我们开发了一种用于通过微阵列检测单核苷酸多态性（SNP）的金属离子螯合辅助连接方法。一个寡核苷酸探针在缺口处被分成两个带有亚氨基二乙酸残基的9至10聚体。只有添加镍离子时，才能与DNA靶标形成双链体，但缺口点对面的核苷酸替换会阻止双链体形成。在此，我们展示了该方法在微阵列上检测5,10-亚甲基四氢叶酸还原酶基因内SNP（A1298C）的应用。