School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Bioorg Med Chem Lett. 2012 Jan 1;22(1):129-32. doi: 10.1016/j.bmcl.2011.11.053. Epub 2011 Nov 20.
Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.
使用带有内部蒽荧光团的寡脱氧核苷酸探针序列，鉴定与前列腺癌相关的基因序列中的单核苷酸多态性。根据合成的靶序列的性质，探针-靶双链体的形成可以导致来自蒽的增强或减弱的荧光发射，从而能够检测到近端碱基对是匹配还是不匹配。
