L-半胱氨酸对强迫游泳所致大鼠脑酶的调节作用。

L-cysteine's effect on modulated rat brain enzymes with forced swimming.

作者信息

Tsakiris Theodore, Angelogianni Panagoula, Tesseromatis Christine, Tsakiris Stylianos, Schulpis Kleopatra H

机构信息

Department of Physiology, Medical School, Athens University, GR-15401 Athens, Greece.

出版信息

Int J Sport Nutr Exerc Metab. 2009 Jun;19(3):285-97. doi: 10.1123/ijsnem.19.3.285.

DOI:10.1123/ijsnem.19.3.285

PMID:19574615

Abstract

BACKGROUND

Forced exercise is associated with oxidative stress, and L-cysteine (L-cys) administration reduces free-radical production.

AIM

To investigate whether L-cys (5 mg/kg) intraperitoneal administration can ameliorate modulated total antioxidant status (TAS), protein concentration, and the activities of acetylcholinesterase (AChE), (Na+,K+)-ATPase, and Mg2+-ATPase in rat brain after 2 and 3 hr of forced swimming.

METHODS

TAS, protein, and enzyme activities were measured spectrophotometrically before and after 2 and 3 hr of exercise without or with L-cys administration.

RESULTS

TAS concentration (55.6 +/- 1.5 vs. 42.1 +/- 1.0 vs. 37.4 +/- 1.2 micromol/L, p < .001), protein concentration (5.68 +/- 0.36 vs. 5.40 +/- 0.18 vs. 4.01 +/- 0.16 mg/ml, p < .01), and AChE activity (0.89 +/- 0.05 vs. 0.61 +/- 0.04 vs. 0.48 +/- 0.03 DeltaOD/min x mg protein, p < .001) were significantly reduced, whereas Na+,K+-ATPase (6.00 +/- 0.36 vs. 10.44 +/- 1.04 vs. 11.90 +/- 1.21 micromol phosphorus inorganic/hr x mg protein, p < .001) and Mg2+-ATPase activity (7.20 +/- 0.65 vs. 10.88 +/- 1.08 vs. 11.55 +/- 1.22 mmol phosphorus inorganic/hr x mg protein, p < .001) were statistically significantly increased after 2 and 3 hr of forced exercise. Post-L-cys administration, AChE activity was decreased (0.90 +/- 0.04 vs. 0.47 +/- 0.02 DeltaOD/min x mg protein, p < .001) and remained unaltered (0.64 +/- 0.04 vs. 0.67 +/- 0.04 DeltaOD/min x mg protein, p > .05) 2 and 3 hr postexercise (0.47 +/- 0.02 vs. 0.54 +/- 0.02 DeltaOD/min x mg protein, p > .05). Na+,K+-ATPase was decreased and remained unchanged (1.85 +/- 0.17 vs. 1.77 +/- 0.19 mumol phosphorus inorganic/hr x mg protein, p > .05) 2 and 3 hr postswimming (1.91 +/- 0.19 vs. 2.06 +/- 0.17 mumol phosphorus inorganic/hr x mg protein, p > .05). Mg2+-ATPase activity was similar with L-cys supplementation pre- vs. postswimming.

CONCLUSIONS

L-cys administration might ameliorate modulated rat brain enzyme activities induced by free-radical production during forced swimming.

摘要

背景

强迫运动与氧化应激相关，而给予L-半胱氨酸（L-cys）可减少自由基生成。

目的

研究腹腔注射L-cys（5mg/kg）能否改善强迫游泳2小时和3小时后大鼠脑内的总抗氧化状态（TAS）、蛋白质浓度以及乙酰胆碱酯酶（AChE）、（Na +，K +）-ATP酶和Mg2 + -ATP酶的活性。

方法

在2小时和3小时运动前后，分别在未给予或给予L-cys的情况下，通过分光光度法测量TAS、蛋白质和酶活性。

结果

强迫运动2小时和3小时后，TAS浓度（55.6±1.5对42.1±1.0对37.4±1.2μmol/L，p<.001）、蛋白质浓度（5.68±0.36对5.40±0.18对4.01±0.16mg/ml，p<.01）和AChE活性（0.89±0.05对0.61±0.04对0.48±0.03ΔOD/min·mg蛋白质，p<.001）显著降低，而Na +，K + -ATP酶（6.00±0.36对10.44±1.04对11.90±1.21μmol无机磷/hr·mg蛋白质，p<.001）和Mg2 + -ATP酶活性（7.20±0.65对10.88±1.08对11.55±1.22mmol无机磷/hr·mg蛋白质，p<.00l）在统计学上显著增加。给予L-cys后，运动后2小时和3小时AChE活性降低（0.90±0.04对0.47±0.02ΔOD/min·mg蛋白质，p<.001）且保持不变（0.64±0.04对0.67±0.04ΔOD/min·mg蛋白质，p>.05）（0.47±0.02对0.54±0.02ΔOD/min·mg蛋白质，p>.05）。游泳后2小时和3小时，Na +，K + -ATP酶降低且保持不变（1.85±0.17对1.77±0.19μmol无机磷/hr·mg蛋白质，p>.05）（1.91±0.19对2.06±0.17μmol无机磷/hr·mg蛋白质，p>.05）。补充L-cys前后Mg2 + -ATP酶活性相似。