Tsakiris T, Angelogianni P, Tesseromatis C, Tsakiris S, Schulpis K H
Department of Experimental Physiology, Medical School, Athens University, PO Box 65257, GR-15401 Athens, Greece.
Br J Sports Med. 2008 May;42(5):367-72. doi: 10.1136/bjsm.2007.039792. Epub 2007 Nov 5.
Forced exercise produces free radicals and L-carnitine (L-C) administration reduces oxidative stress.
To investigate whether short (2 hours) or prolonged (3 hours) forced swimming could modulate total antioxidant status (TAS), protein concentration and activities of acetylcholinesterase (AChE), Na(+)K(+)-ATPase and Mg(2+)-ATPase in rat brain following intraperitonal administration of L-C (300 mg/kg).
TAS, protein and enzyme activities were measured spectrophotometrically.
TAS, protein concentration and AChE activity were reduced, whereas Na(+)K(+)-ATPase and Mg(2+)-ATPase were significantly increased after either 2 or 3 hours of training. L-C administration resulted in a profound restoration of TAS and protein concentration whereas AChE and Na(+)K(+)-ATPase were increased before exercise, followed by AChE restoration and Na(+)K(+)-ATPase reduction after exercise. Mg(2+)-ATPase remained unchanged. An in vitro study using L-C incubation of brain homogenates previously treated with L-C resulted in complete restoration of the modulated enzymes, whereas the enzyme activities from untreated animals remained unaltered.
Short or prolonged swimming in rats may result in a reduction of brain TAS, protein concentration and AChE activity, and an activation of Na(+)K(+)-ATPase and Mg(2+)-ATPase. L-C administration may prevent reduction in TAS and protein concentration, and a decrease in AChE and Na(+)K(+)-ATPase activity; the latter reached pre-exercise values after L-C incubation.
强迫运动产生自由基，而给予左旋肉碱（L-C）可减轻氧化应激。
研究腹腔注射L-C（300mg/kg）后，短时间（2小时）或长时间（3小时）强迫游泳是否能调节大鼠脑内的总抗氧化状态（TAS）、蛋白质浓度以及乙酰胆碱酯酶（AChE）、钠钾ATP酶（Na(+)K(+)-ATPase）和镁ATP酶（Mg(2+)-ATPase）的活性。
采用分光光度法测定TAS、蛋白质和酶活性。
训练2小时或3小时后，TAS、蛋白质浓度和AChE活性降低，而Na(+)K(+)-ATPase和Mg(2+)-ATPase显著增加。给予L-C可使TAS和蛋白质浓度得到显著恢复，而AChE和Na(+)K(+)-ATPase在运动前增加，运动后AChE恢复而Na(+)K(+)-ATPase降低。Mg(2+)-ATPase保持不变。一项体外研究显示，对先前用L-C处理过的脑匀浆进行L-C孵育可使受调节的酶完全恢复，而未处理动物的酶活性保持不变。
大鼠短时间或长时间游泳可能导致脑内TAS、蛋白质浓度和AChE活性降低，以及Na(+)K(+)-ATPase和Mg(2+)-ATPase激活。给予L-C可预防TAS和蛋白质浓度降低，以及AChE和Na(+)K(+)-ATPase活性降低；后者在L-C孵育后达到运动前水平。
