拉莫斯伯基特淋巴瘤细胞系中依赖和不依赖DNA酶γ的凋亡性DNA片段化
DNase gamma-dependent and -independent apoptotic DNA fragmentations in Ramos Burkitt's lymphoma cell line.
作者信息
Mizuta Ryushin, Mizuta Midori, Araki Shinsuke, Suzuki Kohei, Ebara Shota, Furukawa Yuki, Shiokawa Daisuke, Tanuma Sei-ichi, Kitamura Daisuke
机构信息
Division of Molecular Biology, Research Institute for Biological Sciences, Tokyo University of Science, Noda, Chiba, Japan.
出版信息
Biomed Res. 2009 Jun;30(3):165-70. doi: 10.2220/biomedres.30.165.
DNA fragmentation is a biochemical hallmark of apoptosis. Several endonucleases, including CAD/DFF40 and endonuclease G, are implicated in DNA fragmentation. DNase gamma has also been considered to be one of the enzymes involved, but its role in relation to CAD/DFF40 in apoptosis has not been fully elucidated. Here, we distinguished between DNase gamma-dependent and CAD/DFF40-dependent DNA fragmentations. We found that DNase gamma activities appeared in the late apoptotic phase and accelerated DNA fragmentation. Thus, even if the apoptotic DNA fragmentation is initiated by CAD/DFF40, DNase gamma is required for the more complete digestion of the genomic DNA in dying cells.
DNA片段化是细胞凋亡的一个生化标志。包括CAD/DFF40和核酸内切酶G在内的几种核酸内切酶与DNA片段化有关。DNaseγ也被认为是相关酶之一，但其在细胞凋亡中与CAD/DFF40相关的作用尚未完全阐明。在这里，我们区分了DNaseγ依赖性和CAD/DFF40依赖性的DNA片段化。我们发现DNaseγ活性出现在凋亡后期并加速了DNA片段化。因此，即使凋亡性DNA片段化由CAD/DFF40启动，DNaseγ对于垂死细胞中基因组DNA的更完全消化也是必需的。
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