Mizuta Ryushin, Kiku Yoshio, Hayashi Tomohito
Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.
Dairy Hygiene Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-0045, Japan.
J Vet Med Sci. 2018 Aug 22;80(8):1301-1304. doi: 10.1292/jvms.17-0603. Epub 2018 Jun 25.
Cigarette smoke is a strong and independent risk factor for esophageal cancer, while the consumption of cow's milk has been proposed as a protective factor. The mechanistic role of milk in preventing cancer, however, has not been clarified. We focused our study on acrolein, an abundant unsaturated aldehyde present in cigarette smoke. Acrolein is a highly toxic compound and a putative carcinogen. Using a cell culture system, we found that (1) acrolein caused necrosis in Ramos Burkitt's lymphoma cells, (2) the necrosis was inhibited by preincubation of acrolein with milk, and (3) acrolein formed adducts with milk proteins. These results indicated the protective effects of cow's milk against acrolein-induced cytotoxicity via protein-acrolein adduct formation.
香烟烟雾是食管癌的一个强大且独立的风险因素，而饮用牛奶则被认为是一种保护因素。然而，牛奶在预防癌症方面的作用机制尚未明确。我们的研究聚焦于丙烯醛，一种香烟烟雾中大量存在的不饱和醛。丙烯醛是一种剧毒化合物，也是一种潜在致癌物。通过细胞培养系统，我们发现：（1）丙烯醛可导致伯基特氏淋巴瘤细胞坏死；（2）牛奶预孵育可抑制丙烯醛诱导的坏死；（3）丙烯醛与牛奶蛋白形成加合物。这些结果表明，牛奶通过形成蛋白 - 丙烯醛加合物对丙烯醛诱导的细胞毒性具有保护作用。
